Macrophage migration inhibitory factor protects H9c2 cardiac myocytes against hypoxia/reoxygenation injury through regulation of autophagy

doi:10.12092/j.issn.1009-2501.2018.05.008

Abstract

Abstract:

AIM: To investigate the effects of macrophage migration inhibitory factor (MIF) on autophagy of H9c2 cardiac myocytes during hypoxia/reoxygenation (H/R) injury so as to explore the molecular mechanism. METHODS: The MIF mRNA-targeting siRNA was transfected to H9c2 cardiac myocytes. The H/R models of H9c2 cardiac myocytes were established. The autophagy inhibitor 3-methyladenine (3-MA) was added.The levels of MIF, LC3, Cleaved caspase-3 and mTOR protein expression in H9c2 cardiac myocytes were detected by Western blot. RESULTS: MIF siRNA transfection inhibited H/R-induced autophagy. The inhibitor of autophagy 3-MA suppressed H/R-induced autophagy and decreased apoptosis. MIF knockdown increased the expression of p-mTOR during H/R. CONCLUSION: MIF inhibits autophagy in H9c2 cardiomyocytes subjected to H/R, which is related to active mTOR protein.

Key words: macrophage migration inhibitory factor, hypoxia/reoxygenation, H9c2 cardiac myocyte, autophagy, apoptosis

CLC Number:

R965.2

LI Jinyu, HUANG Danmei, ZHANG Yanmei, SHI Ganggang, WANG Bin. Macrophage migration inhibitory factor protects H9c2 cardiac myocytes against hypoxia/reoxygenation injury through regulation of autophagy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(5): 531-535.

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