Abstract:

AIM: To establish an experimental device to test the nicotine concentration of cigarette smoke. METHODS: The nicotine concentration and peak area linear equation were established by GC-MS. It was used to determine the different flow rates of air for the carrier gas purge of liquid nicotine formation of aerosol nicotine concentration. Serum cotinine concentration in rats was determined by ELISA. RESULTS: In the range of 0.226 0 to 2.269 7 μg/mL, the nicotine peak area showed a good linear relationship with its mass concentration, and the correlation coefficient (r²=0.993 7).The average recoveries were 97.2%, 113.7%, and 108.1%, respectively, with standard gaseous nicotine concentrations of 0.567 4, 1.134 8, and 1.702 3 μg/mL, respectively. The relative standard deviations (RSD) were 6.1% and 20.8%,16.9%,respectively (n=3).The retention time of standard and e-cigarette nicotine was 13.41 min. Fragment quantification ion m/z 84.03 and auxiliary quantification ion m/z 133.03 appeared. The specificity of the method was good. Electronic cigarette smoke nicotine concentration was 0.584 μg/mL. Conditions: 25 ℃ nicotine liquid height of 20 cm, air flow 1.1 L/min, the experimental device aerosol nicotine concentration and electronic cigarette smoke matched. Compared with the control group (2.05±0.83) ng/mL, the serum cotinine concentration in the nicotine group was significantly higher(5.31±0.62) ng/mL (P<0.01). CONCLUSION: An experimental device is established to test the nicotine concentration of the electronic cigarette smoke,which can infect rats with nicotine.

Key words: gas purge method, e-cigarette, gas chromatography mass spectrometry (GC-MS), nicotine, cotinine