Lycopene protects against CCl4-induced acute hepatic injury via inhibiting endoplasmic reticulum stress

doi:10.12092/j.issn.1009-2501.2018.07.008

Abstract

Abstract:

AIM: To investigate the effects and mechanism of lycopene on endoplasmic reticulum stress (ERS) in CCl₄-induced acute liver injury mice. METHODS: The mice were treated with lycopene (i.g.) and acute hepatic injury was induced by carbon tetrachloride (CCl₄, i.p.). The activity of lactic dehydrogenase (LDH) in liver tissue was measured by kit. The apoptosis was measured by TUNEL assay. Western blot was performed for proteins. RESULTS: Lycopene decreased the activity LDH (P<0.01) significantly. Meanwhile, lycopene inhibited apoptosis makedly (P<0.05). In addition, pretreatment with lycopene inhibited the expressions of glucose regulated protein 78kda (GRP78), activating trnsfection factor-6 (ATF-6), inositol requiring enzyme (p-IRE-1α), eukaryotic initiation factor-2α (p-eIF-2α) and caspase-12 (P<0.05). Furthermore, the ratio of p-Akt/Akt was down-regulated by lycopene (P<0.05). CONCLUSION: Lycopene alleviates CCl4-induced liver injury by inhibiting ER stress, and the mechanism might be associated with the activation of PI3K/Akt signaling pathway.

Key words: lycopene, acute liver injury, endoplasmic reticulum stress

CLC Number:

R965.2

LIAO Xiaoling，WU Liang，LIN Zongze，YE Jian. Lycopene protects against CCl₄-induced acute hepatic injury via inhibiting endoplasmic reticulum stress[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(7): 770-775.

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Lycopene protects against CCl₄-induced acute hepatic injury via inhibiting endoplasmic reticulum stress

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