Effects of Shenggu zaizao pills on VEGF and FLT-1 expression in steroid-induced avascular necrosis of femoral head in rabbits

doi:10.12092/j.issn.1009-2501.2018.08.006

Abstract

Abstract:

AIM: To explore the intervention mechanism of Shenggu zaizao pills on the steroid induced avascular necrosis of femoral head (SANFH) of rabbit by investigating its effect on vascular endothelial growth factor (VEGF) and its receptor FLT-1. METHODS: Ten Japanese rabbits were randomly selected as blank normal group (group A).The remaining 70 Japanese rabbits were chosen to establish SANFH model, and were administered with low doses of Escherichia coli endotoxin (LPS) combined with high dose of Hormone, four died during the modeling process. Two weeks after establishing model, six Japanese rabbits were randomly selected and sacrificed by air vein injection. HE staining was applied to the pathological sections, and the pathological change of femoral head tissue was observed under high power (×400) optical microscope. When it is confirmed that SANFH model was established successfully, the remaining Japanese rabbits were randomly divided into 4 groups: model group (group B), and low-, medium- and high- dose of Shenggu zaizao pills (group C, D, E), twelveJapanese rabbits in each group. RESULTS:HE pathological sections showed that the number of empty lacuna in B group was the highest at the same time point, and it was more significantly reduced in group C, D and E as compared with group B (P＜0.05)；With time passing，group C, D and E were more significantly reduced as compared with early weeks (P＜0.05). CONCLUSION: Shenggu zaizao pills can promote angiogenesis by up-regulating the expression of VEGF and its receptor FLT-1, which assists the repair of necrotic bone tissue.

Key words: Shenggu zaizao pills, teroid induced avascular necrosis of femoral head (SANFH), VEGF, FLT-1

CLC Number:

R965.2

CAO Linzhong, WANG Xiaomin, ZHANG Hulin, GUO Chenglong, SU Lianglei, LIU Xinyu. Effects of Shenggu zaizao pills on VEGF and FLT-1 expression in steroid-induced avascular necrosis of femoral head in rabbits[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 874-880.

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