Effect of paeonol on the expression of cytokines production and maturation of DCs co-stimulated by FSL-1 and IL-4

doi:10.12092/j.issn.1009-2501.2019.01.003

Abstract

Abstract:

AIM: To study the molecular mechanism of natural paeonol induced by TLR2 signal transduction pathway of inflammation of the skin, developing new ideas for prevention and treatment of atopic dermatitis and provide a theoretical basis. METHODS: Bone marrow-derived dendritic cells (BMDCs) were divided into three groups: control group, FSL-1 stimulated group, paeonol treatment group with different concentrations (25, 50, 100 g/mL). The DC surface molecules such as CD40, CD86 and MHC-II were detected by flow cytometry. The cytokine levels of IL-10, IL-12 in culture supernatant were detected by ELISA. RESULTS: The expression of CD40, CD86 and MHC-II on the surface of dendritic cell surface molecules were significantly increased after stimulated with FSL-1 as compared to control group （P<0.05）. After treated with paeonol (50, 100 g/mL), expressions of CD40, CD86 and MHC-II were all decreased, only in the high and middle dose groups, the difference was statistically significant (P<0.05). Compared with the control group, FSL-1 stimulated group significantly enhanced the secretion of pro-inflammatory cytokines IL-12（P<0.05）, while the anti-inflammatory cytokine IL-10 was decreased significantly （P<0.05）. Meanwhile, compared with FSL-1 stimulated group, the levels of IL-12 were found significantly decreased in the middle and high dose of paeonol treatment group（P<0.05）, but the low dose treatment group did not show significant effect（P>0.05）. The above results showed that FSL-1 can change the differentiation of lymphocyte subset through affecting the secretion of DC cytokines, which can promote the pro-inflammatory cytokines such as IL-12 secretion, while the secretion of anti-inflammatory cytokines such as IL-10 were decreased. But paeonol reversed these effects. CONCLUSION: FSL-1, the TLR2 ligands, can promote the development of chronic dermatitis and chronic inflammation of the development through influencing the expression of cytokines production and maturation of DCs. The level of IL-12 is increased while level of IL-10 is decreased by activating the TLR2 signaling pathway. Furthermore, the primitive Th cells are promoted to transform to Th1 thereby resulting in Th1/Th2 polarization. However, paeonol can reverse FSL-1 induced cellular processes, and shows potential in the prevention and treatment of chronic dermatitis.

Key words: paeonol, atopic dermatitis, dendritic cell, cytokine, Th1/Th2

CLC Number:

HU Yuping, ZHOU Haiyun, HUANG Qiaoling, YAO Yimin. Effect of paeonol on the expression of cytokines production and maturation of DCs co-stimulated by FSL-1 and IL-4[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(1): 14-19.

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