Effects of trans-cinnamaldehyde on proliferation and apoptosis of LOVO cells of colon cancer

doi:10.12092/j.issn.1009-2501.2019.09.006

Abstract

Abstract:

AIM: To explore the effects of trans-cinnamaldehyde on proliferation and apoptosis of LOVO cells of colon cancer. METHODS: The cell proliferation was detected by CCK-8 assay, and clone forming ability of colon cancer cells was detected by colony formation assay. The cell apoptosis was assessed by flow cytometry, and the expression of Bcl-2, Bax, and Caspase-3 was detected via Western blot. RESULTS:Trans-cinnamaldehyde significantly inhibited the proliferation and clone forming ability of the colon cancer cells LOVO in vitro. Compared with the control group, apoptosis rate of LOVO cells was significantly increased in the trans-cinnamaldehyde group (P<0.05). Expressions of Bcl-2 and Caspase-3 were increased in the trans-cinnamaldehyde group, with fragmented Bcl-2. CONCLUSION: The trans-cinnamaldehyde can inhibit cell growth and induce apoptosis of LOVO cells, which involves the up-regulation of Bax and Caspase-3 and the down-regulation of Bcl-2. (E)-cinnamaldehyde is a potnetial anti-tumor target of colon cancer.

Key words: (E)-cinnamaldehyde, colon cancer, proliferation, apoptosis

CLC Number:

ZENG Qiongyao, ZHANG Wenjing, ZHANG Yu, GUO Qiang. Effects of trans-cinnamaldehyde on proliferation and apoptosis of LOVO cells of colon cancer[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(9): 997-1001.

References

［1］张轶雯, 楼倩雯, 钟里科, 等. 基于GEO数据库的近端与远端结肠癌特征基因生物信息学研究［J］. 中国临床药理学与治疗学, 2018, 23(4): 422-427.
［2］毛山山, 程小珍, 崔荣花, 等. 脾多肽注射液联合mFOLFOX6方案治疗晚期结肠癌的效果及对免疫功能的影响［J］. 中国医药导报, 2018, 15(20): 91-95.
［3］袁嘉嘉, 孙志广, 滑永志, 等. “炎癌转化”的病因病机-以慢性萎缩性胃炎为例［J］. 长春中医药大学学报, 2018, 34(4): 18-20.
［4］吴存恩, 王瑞平, 滕钰浩. 肉桂活性成分及抗肿瘤作用研究进展［J］. 时珍国医国药, 2015, 15(8): 1985-1987.
［5］卫高,代立娟,宋英杰.肉桂醛对呼吸道合胞病毒感染宿主HeLa细胞Caspase-9和p-AKT表达的影响［J］.中国老年学杂志,2018,38(9):177-179.
［6］Ali SM,Khan AA,Ahmed I,et al.Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori［J］.Ann Clin Microbiol Antimicrob,2005,43(10):5275-5277.
［7］Somaiya M, Sumayya S, Shafeeque A, et al. Cinnamaldehyde and eugenol attenuates collagen induced arthritis via reduction of free radicals and pro-inflammatory cytokines［J］. Phytomedicine, 2018: S0944-7113(18)30285-X.
［8］Munkhtugs D, Yeon JJ, Geun PH, et al. Studies on the anti-oxidative function of trans-cinnamaldehyde-included β-cyclodextrin complex ［J］. Molecules, 2017, 22(12): 1868.
［9］Meng M, Geng S, Du Z, et al. Berberine and cinnamaldehyde together prevent lung carcinogenesis［J］. Oncotarget, 2017, 8(44): 76385-76397.
［10］王旭林, 王萍, 侯玉龙, 等. 肉桂醛对肝癌HepG2细胞p21和CDK4蛋白的影响［J］. 实用肿瘤杂志, 2016, 10(4): 344-348.
［11］Cabello CM , Bair WB , Lamore SD , et al. The cinnamon-derived Michael acceptor cinnamic aldehyde impairs melanoma cell proliferation, invasiveness, and tumor growth ［J］. Free Radic Biol Med, 2009, 46(2): 220-231.
［12］尹兴忠, 赵冬梅, 刘蕾, 等. 肉桂醛对小鼠U14宫颈癌组织中PI3K表达的影响［J］. 中成药, 2017, 23(1): 194-197.
［13］Arnold M, Mónica SS, Laversanne M, et al. Global patterns and trends in colorectal cancer incidence and mortality［J］. Gut, 2016, 66(4): 683-691.
［14］刘彤, 付彬. 肉桂油五种提取工艺的比较研究［J］. 天津药学, 2018, 20(1): 28-30.
［15］Sima KR, Kanthimathi MS, Nurestri AMS, et al. Cinnamomum cassia suppresses caspase-9 through stimulation of AKT1 in MCF-7 cells but not in MDA-MB-231 cells ［J］. Plos One, 2015, 10(12): e0145216.
［16］冯苏. 肉桂醛对食管癌细胞的作用及其机制研究［D］. 河北：河北医科大学，2017：1-52.
［17］郑晓文, 陈一强, 孔晋亮. 肉桂醛通过Hedgehog信号通路影响人肺腺癌A549细胞的E-cadherin、MMP-9的表达［J］. 中国免疫学杂志, 2014, 20(6): 768-773.
［18］张译之, 时红波, 陈煜. p53凋亡刺激蛋白2的生物学功能及其在肝病中的作用［J］. 临床肝胆病杂志, 2018, 34(11): 187-191.
［19］Abraha AM, Ketema EB. Apoptotic pathways as a therapeutic target for colorectal cancer treatment ［J］. World J Gastrointest Oncol, 2016, 8(8): 583.
［20］李莹莹, 詹丽芬, 曾哲超, 等. 洛铂对肝癌HepG2细胞增殖和调亡的作用及机制［J］. 临床肝胆病杂志, 2018, 34(4): 784-788.
［21］李琪, 任立群, 王亚帝, 等. 芦丁联合奥沙利铂对人胃癌SGC-7901细胞增殖及凋亡的影响［J］. 中国临床药理学与治疗学, 2017, 22(10): 24-30.
［22］Patrick C, Neeraj K, Hina M, et al. Cinnamtannin B-1 inhibits cell survival molecules and induces apoptosis in colon cancer［J］. Int J Oncol, 2018, 53(4): 1442-1454.

[1]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[2]	YANG Xiaojia, JIANG Meng, WU Min, ZHANG Yili, LV Lan, WU Yuanfen, WANG Xinyu, LIU Liquan. Improvement effect of crocin on cognitive impairment of Alzheimer's disease rats through DKK3 regulation of GSK-3 β/β-Catenin pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 489-497.
[3]	WANG Yan, ZHANG Jinhui, ZHAO Yongchen, LIU Hongxiang, LIU Yawei, MIAO Huanhuan, YANG Xincai. Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 508-513.
[4]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[5]	HOU Xiaoyu, LENG Yufang, CAO Xuefen, LV Xingjiao, HAN Xiaoxia, Janvier NIBARUTA, LIU Yongqiang. Network analysis and experimental verification of Schisandrin B reduces intestinal ischemia reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 147-154.
[6]	WANG Jian, SUN Yongdong, ZHOU Xingwei, LIU Lei, CHEN Long, TONG Xingke, ZHU Jiali. Baicalein promotes laryngeal cancer cell death and inhibits invasion via miR-125b-5p/IRF4 axis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1209-1218.
[7]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[8]	ZHOU Huan, XU Ming, LI Bo, LIU Chunying, WANG Chun. PI3K/Akt/FOXO3a signaling pathway inducing protective autophagy promotes acquired lung adenocarcinoma resistance remodeling to DDP [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 961-970.
[9]	HUA Haiyan, YAN Jie, QIN Yufen. Experimental study of schisandrin B attenuates acute myocardial ischemia injury in rats by inhibiting cardiomyocyte apoptosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 848-856.
[10]	DING Jiamin, WANG Youdi, ZHAO Xiali, JIANG Genfeng, CHEN Siwen, HONG Chengcheng, LI Shuqin, HU Weihua. Study on the treatment of thin endometrium with growth hormone and vitamin E [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 857-862.
[11]	LI Xueheng, LI Long. Research progress on the relationship between melatonin and idiopathic pulmonary fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 715-720.
[12]	WANG Xinli, XU Xiaqing, FANG Hanbing, GUO Yuzhong. Study of TPA on enhancing the anti-tumor effects of cisplatin and reducing its renal toxicity [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(5): 535-543.
[13]	YANG Liu, LUO Ailin, LI Shiyong. Advance of general anesthetics-induced developing brain injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(12): 1381-1390.
[14]	TAN Fenglang, REN Hongli, WANG Hongfei. Effect of Phellodendri amurense extract on hippocampal nerve cells induced by Aβ_1-42 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1213-1220.
[15]	LI Yanyan, FANG Xiaojie, YIN Xin, SUN Xi, RAO Chunhui. circZNF124 regulates the proliferation, migration and invasion of colorectal cancer SW620 cells by targeting miR-4262 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1231-1239.