Effect of allicin on apoptosis and Pink1/Parkin signal path of cardiomyocyte in db/db mice

Abstract

Abstract:

AIM: To observe the effect of allicin on apoptosis and Pink1/Parkin signal path of cardiomyocyte in db/db mice. METHODS: Diabetes models were randomly divided into diabetes model group and allicin group. After 21 days of injection, all mice were sacrificed and samples were gathered. Cell apoptosis was analyzed using TUNEL. The mRNA and protein expression level of Pink1, Parkin and Mfn1 were detected by qRT-PCR and Western blot. RESULTS: Severe cardiomyocyte apoptosis, lower expression of Parkin, and higher expression of Mfn1 were observed in diabetes model group, while in allicin group, such changes were improved. CONCLUSION:A llicin can effectively restrain cardiomyocyte apoptosis and protect cardiomyocyte by activating Pink1/Parkin signal path.

Key words: allicin, cardiomyocyte, apoptosis, db/db mice, Parkin

CLC Number:

R965.2

JING Zhe, LIU Fengzhou, GUO Wenyun, JIAO Piqi, CHEN Yongqing. Effect of allicin on apoptosis and Pink1/Parkin signal path of cardiomyocyte in db/db mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(6): 601-605.

References

［1］Di Pino A, Urbano F, Piro S, et al.Update on pre-diabetes: Focus on diagnostic criteria and cardiovascular risk［J］.World J Diabetes, 2016, 7(18):423-432.
［2］Vancampfort D, Mugisha J, Hallgren M, et al.The prevalence of diabetes mellitus type 2 in people with alcohol use disorders: a systematic review and large scale meta-analysis［J］.Psychiatry Res, 2016, 246:394-400.
［3］Yin J, Kong AP, Chan JC. Prevention and care programs addressing the growing prevalence of diabetes in China ［J］.Curr Diab Rep, 2016, 16(12):130.
［4］Zhao YZ, Zhang M, Tian XQ, et al. Using basic fibroblast growth factor nanoliposome combined with ultrasound-introduced technology to early intervene the diabetic cardiomyopathy ［J］. Int J Nanomedicine, 2016,18(11):675-686.
［5］Mizamtsidi M, Paschou SA, Grapsa J, et al. Diabetic cardiomyopathy: a clinical entity or a cluster of molecular heart changes［J］? Eur J Clin Invest, 2016, 46(11):947-953.
［6］Ong SB, Hausenloy DJ. Mitochondrial morphology and cardiovascular disease［J］. Cardiovasc Res, 2010, 88(1):16-29.
［7］An D, Rodrigues B. Role of changes in cardiac metabolism in development of diabetic cardiomyopathy［J］. Am J Physiol Heart Circ Physiol, 2006, 291(4):H1489-1506.
［8］Poole AC, Thomas RE, Andrews LA, et al. The PINK1/Parkin pathway regulates mitochondrial morphology［J］. Proc Natl Acad Sci U S A, 2008,105 (5) 1638-1643.
［9］Deng H, Dodson MW, Huang H, et al. The Parkinson's disease genes pink1 and parkin promote mitochondrial fission and/or inhibit fusion in Drosophila［J］. Proc Natl Acad Sci U S A, 2008, 105 (38)：14503-14508.
［10］Yu W, Gao B, Li N, et al.Sirt3 deficiency exacerbates diabetic cardiac dysfunction: Role of Foxo3A-Parkin-mediated mitophagy ［J］.Biochim Biophys Acta, 2017，1863（8）：1973-1983.
［11］Sheppard JG, Long TE.Allicin-inspired thiolated fluoroquinolones as antibacterials against ESKAPE pathogens［J］.Bioorg Med Chem Lett, 2016, 26(22): 5545-5549.
［12］Hayat S, Cheng Z, Ahmad H, et al.Garlic, from remedy to stimulant: evaluation of antifungal potential reveals diversity in phytoalexin allicin content among garlic cultivars; allicin containing aqueous garlic extracts trigger antioxidants in cucumber ［J］.Front Plant Sci, 2016, 7:1235.
［13］Panyod S, Wu WK, Ho CT, et al. Diet supplementation with allicin protects against alcoholic fatty liver disease in mice by improving anti-inflammation and antioxidative functions［J］.J Agric Food Chem, 2016, 64(38):7104-7113.
［14］Ma LN, Li LD, Li SC, et al. Allicin improves the cardiac function by protecting against apoptosis in rat model of myocardial infarction［J］.Chin J Integr Med, 2016 Jul 13. ［Epub ahead of print］
［15］Huang H, Jiang Y, Mao G, et al. Protective effects of allicin on streptozotocin-induced diabetic nephropathy in rats［J］.J Sci Food Agric,2016, 97(4):1359-1366.
［16］Zou X, Liang J, Sun J, et al.Allicin sensitizes hepatocellular cancer cells to anti-tumor activity of 5-fluorouracil through ROS-mediated mitochondrial pathway ［J］.J Pharmacol Sci, 2016, 131(4):233-240.
［17］李少春,马丽娜,陈坚,等.大蒜辣素对大鼠心肌梗死后纤维化的影响及与TGFβ/Smads信号通路的关系［J］.中国中药杂志, 2016, 41:2517-2521.
［18］史云秋,王旭.从五脏论治2型糖尿病［J］.长春中医药大学学报,2015,31(1):68-70.
［19］Ma LN, Li LD, Li SC, et al. Allicin improves the cardiac function by protecting against apoptosis in rat model of myocardial infarction［J］.Chin J Integr Med, 2016 Jul 13. ［Epub ahead of print］.
［20］Chen X, Pang S, Lin J, et al. Allicin prevents oxidized low-density lipoprotein-induced endothelial cell injury by inhibiting apoptosis and oxidative stress pathway ［J］.BMC Complement Altern Med, 2016, 16:133.
［21］Boudina S, Abel ED. Mitochondrial uncoupling: a key contributor to reduced cardiac efficiency in Diabetes［J］. Physiology (Bethesda), 2006, 21:250-258.
［22］De Rosa A, Pellegrino T, Pappatà S, et al.Myocardial 123I-metaiodobenzylguanidine scintigraphy in patients with homozygous and heterozygous parkin mutations［J］.J Nucl Cardiol,2015, 24(1):103-107.
［23］Santel A, Fuller MT. Control of mitochondrial morphology by a human mitofusin［J］. J Cell Sci, 2001, 114(Pt 5):867-874.
［24］Tanaka A, Cleland MM, Xu S, et al. Proteasome and p97 mediate mitophagy and degradation of mitofusins induced by Parkin［J］. J Cell Biol, 2010,191(7) 1367-1380.
［25］Bhandari P, Song M, Chen Y, et al. Mitochondrial contagion induced by Parkin deficiency in Drosophila hearts and its containment by suppressing mitofusin［J］. Circ Res,2014,114 (2):257-265.

[1]	WANG Jingang, LI Qiang, SUN Huiyan, WANG Hongquan. Recent progress in understanding the neuroprotective mechanism of carnosic acid in Parkinson's disease [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(9): 1073-1080.
[2]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[3]	YANG Xiaojia, JIANG Meng, WU Min, ZHANG Yili, LV Lan, WU Yuanfen, WANG Xinyu, LIU Liquan. Improvement effect of crocin on cognitive impairment of Alzheimer's disease rats through DKK3 regulation of GSK-3 β/β-Catenin pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 489-497.
[4]	ZHANG Xuejiao, LIU Jieting, LI Luxin, CHEN Peijian, DING Minglu, SUN Mengwei, CHU Yanhui, ZHANG Zhen . Effects and mechanism of dapagliflozin on myocardial injury in type 1 diabetes mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(3): 257-265.
[5]	HOU Xiaoyu, LENG Yufang, CAO Xuefen, LV Xingjiao, HAN Xiaoxia, Janvier NIBARUTA, LIU Yongqiang. Network analysis and experimental verification of Schisandrin B reduces intestinal ischemia reperfusion injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(2): 147-154.
[6]	WANG Jian, SUN Yongdong, ZHOU Xingwei, LIU Lei, CHEN Long, TONG Xingke, ZHU Jiali. Baicalein promotes laryngeal cancer cell death and inhibits invasion via miR-125b-5p/IRF4 axis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1209-1218.
[7]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[8]	ZHOU Huan, XU Ming, LI Bo, LIU Chunying, WANG Chun. PI3K/Akt/FOXO3a signaling pathway inducing protective autophagy promotes acquired lung adenocarcinoma resistance remodeling to DDP [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(9): 961-970.
[9]	HUA Haiyan, YAN Jie, QIN Yufen. Experimental study of schisandrin B attenuates acute myocardial ischemia injury in rats by inhibiting cardiomyocyte apoptosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 848-856.
[10]	LI Xueheng, LI Long. Research progress on the relationship between melatonin and idiopathic pulmonary fibrosis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 715-720.
[11]	YANG Liu, LUO Ailin, LI Shiyong. Advance of general anesthetics-induced developing brain injury [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(12): 1381-1390.
[12]	TAN Fenglang, REN Hongli, WANG Hongfei. Effect of Phellodendri amurense extract on hippocampal nerve cells induced by Aβ_1-42 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1213-1220.
[13]	PAN Rongrong, ZHI Yinghao, JIN Yongxi, ZHOU Xiahui. Influences of theaflavins on neuronal apoptosis and blood-brain barrier in rats with cerebral hemorrhage by regulating CaMKK2/AMPK signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1240-1246.
[14]	LI Ying, YAO Wei, WANG Meng, YU Zhihong, GONG Yuanqi, LAN Haibing, QI Xiefei. Experimental study of irisin alleviates house dust mite-induced airway epithelial cells inflammation and apoptosis via the NF-κB and JNK signaling pathways [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(10): 1106-1112.
[15]	FAN Wenxiang, ZHANG JinLu, XU Chi. Research progress of α7 nicotinic acetylcholine receptors in central nervous system diseases [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(9): 1065-1072.