Effect and mechanism of total flavones of rhododendra on membrane hyperpolarization in rat hippocampal neurons and EA.hy926 cells

Abstract

Abstract:

AIM: To observe the effect and mechanism of total flavones of rhododendra (TFR) on membrane hyperpolarization in rat hippocampal neurons and EA.hy926 vascular endothelial cells. METHODS: Rat hippocampal neurons and EA.hy926 cells were cultured in vitro; the changes in fluorescence intensity of endothelial cells and rat neurons were measured by DiBAC4(3) fluorescent dyes through the calcium ion imaging system with the excitation wavelength of 488 nm to reflect the membrane potential.RESULTS:Compared with control group, TFR (270 mg/L and 810 mg/L) could markedly reduce intensity of fluorescence in EA.hy926 cells, and the effect could be inhibited by BKCa specific inhibitor IbTX, the co-application of IKCa inhibitor ChTX and SKCa inhibitor apamin, while DL-propargylglycine（PPG） couldn't;Compared with Control group, TFR (270 mg/L and 810 mg/L) could obviously reduce the intensity of fluorescence in rat hippocampal neurons and this effect could also be blocked by IbTX.CONCLUSION: TFR can cause membrane hyperpolarization in EA.hy926 cells and rat hippocampal neurons; the possible mechanism is related to activating KCa channels.

Key words: total flavones of rhododendra, endothelial cells, hippocampal neurons, Ca ²⁺ -sensitive K+ channels, cell membrane potential

CLC Number:

R965.2

ZHANG Jie, CHEN Zhiwu. Effect and mechanism of total flavones of rhododendra on membrane hyperpolarization in rat hippocampal neurons and EA.hy926 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(6): 606-610.

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