Effect of volatile oil of the angelica combined with carboplatin on the proliferation and apoptosis of human lung adenocarcinoma cell A549

Abstract

Abstract:

AIM: To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell A549 combined with carboplatin. METHODS: Use the MTT colorimetric method to investigate cytotoxicity effect of volatile oil of the angelica alone or combined with carboplatin to human lung adenocarcionma cell A549; Use flow cytometry（FCM）analysis the cell apoptosis. Observe the cell apoptosis by AO/BE fluorescent staining method. RESULTS: The A549 cell was inhibited by volatile oil of the angelica alone or combined with carboplatin in a concentration-dependent manner, and compared with use volatile oil of the angelica alone, the IC50 descend in drug combination group. Results of flow cytometry indicates that, volatile oil of the angelica alone or combined with carboplatin, could induce apoptosis and necrosis of A549 cell, and compared with use volatile oil of the angelica alone, the rate of apoptosis and necrosis all rise in drug combination group. The results of AO/BE fluorescent staining method displayed that, the rate of apoptosis and necrosis were all raised in drug combination group. CONCLUSION: Volatile oil of the angelica had depressant effect on the generation of A549 cell, there is a synergistic cytotoxic effect on A549 cells treated with volatile oil of the angelica combined with carboplatin. The mechanism is probably that it can induce apoptosis of A549 cell.

Key words: volatile oil of the angelica, carboplatin, human lung adenocarcinoma cell A549, apoptosis

CLC Number:

R965.2

ZHANG Yanxia, ZHANG Zhengshun, CHEN Shaoyi, KONG Xueli, ZHOU Jiawei. Effect of volatile oil of the angelica combined with carboplatin on the proliferation and apoptosis of human lung adenocarcinoma cell A549[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 755-761.

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