Abstract:

AIM: To investigate the hypoglycemic effect and inhibition of alpha glucosidase activity in vitro of three polysaccharide obtained from ethanol fractionation precipitation on diabetic mice constructed with streptozotocin (STZ).  METHODS: Three polysaccharides were obtained by fractionation and precipitation of total polysaccharides with ethanol. The diabetic mice were constructed by intraperitoneal injection of STZ and divided into control group, model group, drug group (divided into three groups, three kinds of polysaccharides). Fourteen days after drug intervention, the fasting blood glucose (FBG), insulin (FINS), glucagon (GC), expression of insulin resistance index (HOMA-IR), SOD, MDA and GSH-Px in pancreatic islets of mice were detected. RESULTS: Three kinds of polysaccharides had hypoglycemic effects at different degrees, and their effects were related to the expression of insulin and glucagon. Polysaccharides also interfered with the expression of SOD, MDA and GSH-Px in islet tissues at different degrees. In vitro studies showed that polysaccharides significantly inhibited alpha glucosidase and the effects were dose-dependent. CONCLUSION: The polysaccharide has significant therapeutic effect on diabetic mice, but the effects of different components of polysaccharides are different, and the mechanism is diverse. Antrodia camphorate polysaccharide is a promising polysaccharide drug, which deserves further study and development.

Key words: polysaccharides, diabetic mice, alpha glucosidase