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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2018, Vol. 23 ›› Issue (6): 608-613.doi: 10.12092/j.issn.1009-2501.2018.06.002

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Protective effects of β-asarone on PC12 cells injury induced by Aβ1-42 activated ACM

HE Ying1, BAO Yuting 2, YAO Ying 3, XUAN Ling2, YANG Yuanxiao3   

  1. 1 Hangzhou Red Cross Hospital, Hangzhou 310003, Zhejiang, China; 2 Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China; 3 Hangzhou Medical Collage, Hangzhou 310053, Zhejiang, China
  • Received:2017-09-18 Revised:2017-10-11 Online:2018-06-26 Published:2018-06-19

Abstract:

AIM: To investigate the effect of different concentrations of Aβ 1-42 activated-ACM cell on the viability and expression of BDNF, AChE of PC12 cells, and to explore the protective effects of β-asarone on the PC12 cells. METHODS: The logarithmic growth PC12 cells were randomly divided into normal group, ACM (Aβ1-42 3.3 μmol/L) group, ACM (Aβ1-42 10 μmol/L) group and ACM (Aβ1-42 30 μmol/L) group. The Real time cell analysis (RTCA) system and MTT assay were used to detect the survival rate of PC12 cells, respectively; Western blot assay was used to detect the expression of BDNF, AChE. The logarithmic growth PC12 cells divided into normal group, ACM (Aβ1-42 μmol/L) group, β-asarone(18.5 μg/mL, 55.5 μg/mL, 166.7 μg/mL) group randomly. The change of survival rate of PC12 cells was detected by RTCA assay; the expression of BDNF, AChE were detected by Western blot. RESULTS: There was no significant difference in the survival rate of PC12 cells in each group after ACM activated for 24 h (P>0.05); while the survival rate of PC12 cells was significantly reduced in ACM (Aβ1-42 10 μmol/L) group and ACM (Aβ1-42 30 μmol/L) group (P<0.01) after ACM activated for 36 h; the expression of BDNF, AChE protein increased significantly with the increase of the concentration of Aβ1-42 (P<0.05 or P<0.01); the survival rate of PC12 cells were significantly increased with β-asarone (18.5 μg/mL, 55.5 μg/mL, 166.7 μg/mL); the expression of AChE in β-asarone (55.5 μg/mL, 166.7 μg/mL) group was significantly lower than that in the model group (P<0.05 or P<0.01); the expression of BDNF in β-asarone (55.5 μg/mL) group was increased compared with that of the model group (P<0.05). CONCLUSION: β-asarone can inhibit the increase of AChE expression, and promote the expression of BDNF, suggesting that β-asarone has a certain protective effect on neurons.

Key words: β-asarone, 1-42 , ACM, PC12 cells, BDNF, AChE

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