miR-1304 inhibits non-small cell lung cancer cell invasion and proliferation in vitro via targeting heme oxygenase-1

doi:10.12092/j.issn.1009-2501.2019.04.004

Abstract

Abstract:

AIM:To investigate the direct target of miR-1304 and its function in NSCLC in vitro. METHODS: Real-time quantitative PCR was used to detect the expression of miR-1304 in lung cancer cell A549 and NCI-H1975 .Transwell assay was used to test the role of miR-1304 on regulating invasion and migration of cells. The cell proliferation and survival were investigated via cell counting, MTT and colony-formation assays. Cell apoptosis and cell cycle were examined using annexin V-PE/7-AAD and PI staining assays, respectively. Dual luciferase reporter gene was used to analyze the binding between miR-1304 and 3'UTR of heme oxygenase-1, Western blot detected the HO-1 protein levels. RESULTS:MiR-1304 significantly decreased the number and viability of NSCLC cells and colony formation, and induced cell apoptosis and G0/G1 phase cell cycle arrest. HO-1 was demonstrated to be a direct target of miR-1304 in NSCLC cells. Furthermore, altered expression of miR-1304 by transfection of pre-miR-1304 mimics and inhibitor signifcantly affected the ability of invasion and proliferation of lung cancer cells. Altered expression of miR-1304 markedly down-regulated the HO-1 protein levels of lung cancer cells. In addition, dual luciferase reporter gene assay indicated that miR-1304 regulated HO-1 expression by binding to the 3'UTR of HO-1 mRNA. CONCLUSION: MicroRNA-1304 is a tumor suppressor and HO-1 is its direct target in NSCLC. The results suggest the potential for miR-1304 as a therapeutic target for NSCLC.

Key words: NSCLC, miR-1304, heme oxygenase-1, invasion, proliferation

CLC Number:

R965.2

Yuan ShaoFei. miR-1304 inhibits non-small cell lung cancer cell invasion and proliferation in vitro via targeting heme oxygenase-1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 383-390.

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