Raddeanin inhibits the invasion and metastasis of HEC-1-B cells in human endometrial carcinoma by inhibiting the JAK/STAT3 signaling pathway

doi:10.12092/j.issn.1009-2501.2019.04.005

Abstract

Abstract:

AIM: To investigate the effect of raddeanin on the invasion and metastasis of human endometrial carcinoma HEC-1-B cells, and the effects of the JAK/STAT3 signaling pathway. METHODS: HEC-1-B cells were selected from human endometrial carcinoma in logarithmic phase. One group was treated with raddeanin, which was the observation group. The other group was treated with saline as a control group. The scratch test was used to detect the cell migration at different time points in the two groups. The invasiveness of cells in two groups was compared by Transwell. The proliferation capacity of cells in two groups was measured by CCK-8. The expression of JAK and STAT3 in cells was detected by fluorescence quantitative PCR and Western blot. RESULTS:After 48 h, the migration distance of the observation group was shorter than that of the control group (P<0.05). The number of cells invading the lower compartment was less than that of the control group (P<0.05). The differences in cell proliferation between the two groups were not statistically significant during 48 h（P>0.05）. Compared with the control group, the results of fluorescence quantitative PCR and Western blot showed decreased expression of JAK and STAT3 genes in the observation group（P<0.05）. CONCLUSION: Raddeanin can significantly inhibit the invasion and metastasis of human endometrial carcinoma HEC-1-B cells and its mechanism may be through regulating the JAK/STAT3 signaling pathway.

Key words: raddeanin, JAK, STAT3, HEC-1-B cell, invasion and metastasis

CLC Number:

CHEN Yuequn, ZHAI Hongbo, TONG Jinyi, QU Wanglei. Raddeanin inhibits the invasion and metastasis of HEC-1-B cells in human endometrial carcinoma by inhibiting the JAK/STAT3 signaling pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 391-396.

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