Effect of genistein on cartilage mediated by JAK2/STAT3 signaling pathway in rats with osteoarthritis

doi:10.12092/j.issn.1009-2501.2018.04.004

Abstract

Abstract:

AIM: To investigate the effect of genistein on cartilage and its relation to JAK2/ STAT3 signaling pathway in rats with osteoarthritis（OA）. METHODS: SD rats were randomly divided into control group, OA group and genistein with OA group. After 6 weeks of treatment, the histological morphology of cartilage specimen was observed by Histologic staining; the expression of TNF-α in synovial fluid of rats was detected by ELISA and the expressions of p-JAK2, p-STAT3, MMP-13, Bax and Bcl-2 in articular chondrocytes were detected by Western blot. RESULTS: Genistein can effectively increase the content of collagen and proteoglycan in the OA articular cartilage, and make the cartilage structure close to the normal cartilage. Compared with OA group, the expression of TNF-α in genistein+OA group was significantly down regulated (P<0.01), compared with the normal group, there was no significant difference in TNF-α expression of OA+genistein (P>0.05). Compared with OA group, the expression level of p-JAK2, p-STAT3 and Bcl-2 protein in genistein+OA group increased significantly (P<0.05), and the expression level of MMP-13 and Bax decreased significantly (P<0.05). CONCLUSION: JAK2/STAT3 signaling pathway is closely related to the pathological process of cartilage degeneration in osteoarthritis. Genistein can activate JAK2 STAT3 signaling pathway, inhibit the inflammation of osteoarthritis and the apoptosis of chondrocytes and relieve the degeneration of articular cartilage as well as reduce the level of OA progress.

Key words: osteoarthritis, genistein, JAK2/STAT3 signaling pathway, inflammatory reaction, apoptosis

CLC Number:

R965.2

HU Jiong, WANG Weidong, WANG Changxing, DONG Liqiang, LIU Qiming. Effect of genistein on cartilage mediated by JAK2/STAT3 signaling pathway in rats with osteoarthritis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 383-388.

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