Expression of miR-34a-5p in bladder cancer and its effect on invasion and migration of bladder cancer cells

doi:10.12092/j.issn.1009-2501.2019.04.007

Abstract

Abstract:

AIM: To study the expression of miR-34a-5p in bladder cancer and its effect on invasion and migration of bladder cancer cells. METHODS: 50 cases of bladder cancer and corresponding adjacent tissues were collected, the expression of miR-34a-5p was detected by qRT-PCR. The miR-34a-5p mimics were transfected into bladder cancer cells. The levels of miR-34a-5p, the number of invasion and migration, and the expression levels of E-cadherin, N-cadherin and Vimentin were detected by qRT-PCR, Transwell chamber and Western blot, respectively. Target gene prediction software found that TPD52 might be the target gene of miR-34a-5p; luciferase reporter vector was constructed to identify the target gene. The effect of miR-34a-5p mimics on the expression of TPD52 in bladder cancer cells were detected by Western blot. The expression of TPD52 in bladder cancer and adjacent tissues were detected by qRT-PCR, and the correlation between the expression of TPD52 and miR-34a-5p in bladder cancer were analyzed. The miR-34a-5p mimics and pcDNA3.1-TPD52 were co-transfected into bladder cancer cell. TPD52 protein, number of invasion and migration, and expression of E-cadherin, N-cadherin and Vimentin were detected by the above methods. RESULTS:The expression of miR-34a-5p in bladder cancer tissue was decreased. miR-34a-5p mimics increased the expression level of miR-34a-5p in bladder cancer cells, reduced the invasion and migration ability, reduced the expression of N-cadherin and Vimentin, increased the expression of E-cadherin protein. miR-34a-5p regulated the expression of TPD52, moreover, miR-34a-5p mimics inhibited the expression of TPD52 protein in cells. TPD52 was highly expressed in bladder cancer, which was negatively correlated with the expression level of miR-34a-5p in bladder cancer tissue. Compared with co-transfected cells of miR-34a-5p mimics and pcDNA3.1, co-transfection of miR-34a-5p mimics and pcDNA3.1-TPD52 enhanced the expression of TPD52 in bladder cancer cells, increased the invasive and migratory ability of bladder cancer cells, and promoted the expression of N-cadherin and Vimentin, reduced the expression of E-cadherin protein. CONCLUSION: The expression of miR-34a-5p in bladder cancer tissue was low. Upregulation of miR-34a-5p targeting TPD52 inhibits the invasion and migration of bladder cancer cells.

Key words: bladder cancer, miR-34a-5p, invasion, TPD52

CLC Number:

R737.14

LI Peng, YANG Ronghua, ZHANG Minghua, XIAO Xin, TANG Jianer. Expression of miR-34a-5p in bladder cancer and its effect on invasion and migration of bladder cancer cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 403-410.

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