Expression of miR-19b in osteosarcoma and its effect on invasion and migration of osteosarcoma Saos-2 cells by regulating KLF13

doi:10.12092/j.issn.1009-2501.2019.11.005

Abstract

Abstract:

AIM: To study the expression of miR-19b in osteosarcoma and its effect on invasion and migration of osteosarcoma by regulating KLF13. METHODS: Realtime PCR was used to detect the expression of miR-19b in osteosarcoma tissues and corresponding normal adjacent tissues.miR-19b inhibitor was transfected into osteosarcoma Saos-2 cells. Transwell chamber was used to detect changes in invasion and migration ability.Western blot was used to detect the difference of MMP-9 and MMP-2 protein expression.Target gene prediction software predicts that KLF13 may be a target gene of miR-19b. Luciferase reporting system was used to identify the targeting relationship.Realtime PCR was used to detect the difference of KLF13 expression between osteosarcoma tissues and corresponding normal adjacent tissues. KLF13 siRNA and miR-19b inhibitor were co-transfected into osteosarcoma cells, the differences of cell invasion, migration and expression of MMP-9 and MMP-2 were detected by the above methods.RESULTS:The expression of miR-19b in osteosarcoma tissues was higher than that in adjacent tissues. In osteosarcoma cells transfected with miR-19b inhibitor, the level of miR-19b decreased, cell invasion, migration and the levels of MMP-9 and MMP-2 protein decreased.miR-19b targeting negatively regulates the expression of KLF13. The expression of KLF13 in osteosarcoma was lower than that in normal adjacent tissues, the expression level of miR-19b was negatively correlated with that of miR-19b. KLF13 siRNA can reverse the inhibitory effect of miR-19b inhibitor on invasion, migration and expression of MMP-9 and MMP-2 in osteosarcoma cells.CONCLUSION: miR-19b is highly expressed in osteosarcoma, downregulation of KLF13 expression can inhibit the invasion and migration of osteosarcoma Saos-2 cells by targeting KLF13 expression.

Key words: osteosarcoma, miR-19b, KLF13, invasion, migration

CLC Number:

R965.2
R73

QIAN Wanfeng, WANG Xiufeng, CHEN Xuepeng. Expression of miR-19b in osteosarcoma and its effect on invasion and migration of osteosarcoma Saos-2 cells by regulating KLF13[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1242-1248.

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