Abstract:

AIM: To explore the effect of Fuzheng Kangfu mixture combined with cisplatin on promoting apoptosis and inhibiting proliferation of gastric cancer cell. METHODS: Twenty-eight nude mice were randomly divided into 4 groups as follows: the model control group, the group of Fuzheng, the cisplatin group, the group of cisplatin combined with Fuzheng. There were 7 nude mice in each group. The transplanted gastric cancer model of mice was established by subcutaneous injection of gastric cancer SCG7901 cell line. The Cisplatin as well as Fuzheng were administered drugs continuously for 7 days. The weight and tumor weight were measured and the tumor inhibition rate was calculated .The PCR and immunohistochemistry was applied to detect the apoptosis target of Bcl-2, Bax and Survivin as well as VEGF in nude mice. RESULTS:Compared with the model control group, the group of Fuzheng, the cisplatin group, the group of cisplatin combined with Fuzheng, the tumor weights of all the other nude mice were alleviated in different degrees (P<0.05). Among these, the tumor inhibition rate of the group of Cisplatin combined with Fuzheng was the highest (66.67%). In terms of cell inhibition, Fuzheng Kangfu mixture combined with cisplatin could significantly enhance the expression of Bax, and reduce the expressions of Bcl-2, Survivin, and vascular endothelial growth factor (VEGF) (P<0.05).CONCLUSION: Fuzheng Kangfu mixture combined with cisplatin has the effect of inhibiting tumor proliferation.

Key words: gastric cancer, Fuzheng Kangfu mixture, cisplatin, apoptosis