Effect of JiSuiKang on the microglia infiltration and neuron survival in the spinal cord injury area of rats

doi:10.12092/j.issn.1009-2501.2020.02.006

Abstract

Abstract: AIM: To observe the effect of Chinese medicine compound JiSuiKang on the microglia infiltration and neuron survival at the spinal cord injury area of rats. METHODS: Sixty SD rats were randomly divided into sham operation group (Sham), model group (Ctrl), JSK low (JSK-L), JSK medium (JSK-M) and JSK high (JSK-H) dose group, prednisone group. The model of rat spinal cord injury (SCI) was established by improved Allen's method. After the operation, each group was intervened in different ways, and then we assessed the double hind limb motor function score in rats (BBB score). The heart perfusion was done and the spinal cord tissues from the injury area were taken. ELISA tests were used to detect the expression of inflammatory factors. The spinal cord tissue was sliced into sections and then immunofluorescence stained of CD11b/c and beta 3-Tubulin to observe the infiltration of microglia and the growth of neurons under the inverted fluorescence microscope. RESULTS:The BBB score of Sham, JSK-L, JSK-M, JSK-H and prednisone group were higher than Ctrl（P＜0.05）. For the expression of inflammation, the expressions of IL-1 in group Sham, JSK-L, JSK-M, JSK-H and prednisone were lower than that of Ctrl (P＜0.05). For the percentage of microglia infiltration, JSK-L, JSK-M, JSK-H and prednisone were all lower than that of Ctrl (P＜0.05), and there was no significant difference between JSK-H and prednisone(P＞0.05). For the growth state of the neuron, the JSK-L, JSK-M, JSK-H, prednisone group were better than Ctrl (P＜0.01), and JSK-H was better than JSK-L and JSK-M (P＜0.05). CONCLUSION: The Chinese medicine compound JSK inhibits the migration and invasion of microglia in SCI, promotes the recovery of damaged neurons and motor function in SCI rats.

Key words: JiSuiKang, spinal cord injury, microglia, neurons, migration, infiltration

CLC Number:

PAN Yalan, YUAN Wenchao, MA Yong, GUO Yang, ZHENG Suyang, XU Guihua, LIU Jiahuan. Effect of JiSuiKang on the microglia infiltration and neuron survival in the spinal cord injury area of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(2): 151-158.

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