Inhibition of fenofibrate on angiotensinⅡ-induced high expression of osteopontin in cardiac fibroblasts

Abstract

Abstract: AIM: To investigate the effects of fenofibrate, peroxisome proliferator-activated receptor α (PPARα) agonist, on the up-regulation of angiotensin II (Ang II)-induced osteopontin in rat cardiac fibroblasts. METHODS: Isolated cardiac fibroblasts of neonatal Sprague-Dawley rat were pretreated with various concentrations of fenofibrate (0, 25, 50 and 100 μmmol/L) for 1 hour. Then Ang II were added into cells for 24 hours. The expression of osteopontin was measured by real-time quantitative RT-PCR and Western blotting, separately. RESULTS: Significant inhibitory effects of fenofibrate on Ang II-induced high expression of osteopontin were observed in cardiac fibroblasts and the effect was dose dependent (P<0.05). CONCLUSION: The present data suggests that fenofibrate can down-regulate the level of osteopontin in rat cardiac fibroblasts, which may explain the anti-inflammatory and anti-fibrosis effects of fenofibrate in the cardiovascular system.

Key words: Myocardial fibrosis, Cardiac fibroblast, Fenofibrate, Osteopontin

CLC Number:

R965.2

LI Wen-ju, SHI Miao-qian, OU Dong-bo, DING Lu, LIU Xiao-lin, NIU Xiong-tao, ZHENG Qiang-sun. Inhibition of fenofibrate on angiotensinⅡ-induced high expression of osteopontin in cardiac fibroblasts[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(11): 1211-1215.

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