Effect of Mistletoe Lectins on immunological roles of peritoneal macrophages by intraperitoneal administration

Abstract

Abstract: AIM: To investigate the effect of peritoneal macrophages in mice on tumoricidal activity against colon cancer HCT116, following intraperitoneal injection of Mistletoe Lectins. METHODS: 16 mice were divided into control group and intraperitoneal chemotherapy group. The number of peritoneal macrophages was counted by trypan blue exclusion. The amount of TNF-α and NO expressed by peritoneal macrophages was measured by ELISA and Griess, respectively. The cytotoxity of peritoneal macrophages supernatant against HCT116 was evaluated by MTT assay. RESULTS: It was found that the amount of peritoneal macrophages was increased, the expressions of TNF-α and NO were increased and the cytotoxicity against HCT116 in vitro was increased after intraperitoneal injection of Mistletoe Lectins. CONCLUSION: Intraperitoneal injection of Mistletoe Lectins can activate the peritoneal macrophages in mice and enhance the cytotoxity of the supernatant against HCT116 in vitro.

Key words: Colorectal neoplasm, Mistletoe Lectins, Macrophage, Intraperitoneal chemotherapy

CLC Number:

R965.2

YAN Mei-di, YE Meng, LIN Lei, NI Shu-min, WANG Shao-min, LIU Shu-rong. Effect of Mistletoe Lectins on immunological roles of peritoneal macrophages by intraperitoneal administration[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(2): 126-129.

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