Dynamic change of ERKmRNA and phosphorylation protein level in rats with asthma

Abstract

Abstract: AIM: To explore the dynamic changes of ERKmRNA and phosphorylation protein level in rats with asthma. METHODS: The rats were divided into control group,asthma (0.5,1,3,12 h)groups and the peripheral blood mononuclear cells(PBMCs)of rats were cultured and collected. The expressions of ERKmRNA and phosphor protein of ERK in PBMCs of all groups were detected by RT-PCR,Western bloting, respectively. RESULTS: Compared with control group,the expressions of ERKmRNA,phosphor protein of ERK in PBMCs were increased in asthma (0.5,1,3 )groups (all P<0.01). There was no difference between asthma(12 h)and control group. Compared with control group,asthma (0.5,3,12 h)groups,the expressions of ERKmRNA,phosphor protein of ERK in PBMCs were increased in asthma(1 h)group. CONCLUSION: Phosphorylation ERK may play an important role in airway inflammation and remodeling of asthma,deeply studying the ERK's specific regulation results in the pathogenesis of asthma may have an important significance on the prevention and treatment clinical asthma.

Key words: Asthma, Peripheral blood mononuclear cells

CLC Number:

R965.2

ZHANG Jin-bo, WU Cheng-yun, LI Zhi-qiang, DAI Yuan-rong. Dynamic change of ERKmRNA and phosphorylation protein level in rats with asthma[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(2): 166-169.

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