Abstract: AIM: In order to discuss the relation between clinical adverse reaction and gene polymorphism of CYP1A2、UGT1A7 when using genistein. METHODS: 114 cases of healthy volunteers were randomly divided into 2 groups: model group, and normal group.the model group take genistein 50, 100, 200 mg by oral, and each dose of genistein was observed 3 days for the occurrence of adverse reaction.Gene fragment was amplified by restriction fragment length polymorphism polymerase chain reaction(RFLR-PCR), and polymorphism of CYP1A2G2964A, C734A and UGT1A7Trp208Argwas analysised by enzyme electrophoresis.RESULTS: There had no significant difference of gene distribution between model and normal groups(P>0.05).Model group was divided into 2 groups of genotypes according to adverse reaction occurrence, CYP1A2G2964A gene: the 10 cases genotype among 14 cases in adverse reaction group was G/A(account 71.43%), but 22 cases genotype among 41 cases in no adverse reaction was G/G (account 53.66%); CYP1A2C734A gene: 7 cases genotype among 13 cases in adverse reaction was C/A(account 53.85%), but 16 cases genotype among 32 cases in no adverse reaction was A/A(account 50.00%).UGT1A7Trp 208Arg Gene: 12 cases genotype among 15 cases in adverse reaction was Trp/Trp (account 80.00%), and 32 cases genotype among 53 cases in no adverse reaction was Trp/Trp (account 60.38%).CONCLUSION: The relation gene in adverse reaction by using genistein is CYP1A2G2964A, CYP1A2C734A and UGT1A7, especially higher in G/A, C/A and Trp/Trp for each gene.

Key words: genistein, adverse reaction, CYP1A2, UGT1A7gene polymorphism