Comparison of inhibitory effect of five natural medicines on proliferation induced by rhbFGF in lens epithelial cell

Abstract

Abstract: AIM: To compare the inhibitory effect of five natural medicines on proliferation induced by recombinant human basic fibroblast growth factor (rhbFGF) in lens epithelial cell (LEC). METHODS: The proliferations of cultured LEC were induced by rhbFGF, which were incubated with five natural medicines, [curcumine (Cur), elemene(Ele), arsenite trioxide (As₂O₃ ), triptolide (Tri) and rhizoma curcumae (Rc) ], respectively.The LEC activities were detected via methyl thiazolyl tetrazolium (MTT).The proliferating cell nuclear antigen (PCNA) of LEC were detected via flow cytometer(FCM). RESULTS: The activity of LEC and the expression of PCNA in proliferation group were higher than those in control group (P <0.01). Compared with the proliferation group, the activity of LEC and the expression of PCNA were significantly decreased in group Cur, Ele, As₂O₃, Tri and Rc (P < 0.01).The inhibitory effects of Cur and Ele are the strongest.As₂O₃, in the middle.Tri and Rc are the weakest(P <0.01), the effects of Cur and Ele are similar. CONCLUSION: The five natural medicines can inhibit the proliferation of LEC induced by rhbFGF.Cur and Ele may become effective medicines of preventing and treating after-cataract.

Key words: curcumine, elemene, arsenite trioxide, triptolide, rhizoma curcumae, lens epithelial cell, recombinant human basic fibroblast growth factor, proliferation, after-cataract

CLC Number:

R965.2

QI Ming-xin, ZHANG Qing-lin, HUANG Xiu-rong, YANG Xin. Comparison of inhibitory effect of five natural medicines on proliferation induced by rhbFGF in lens epithelial cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(11): 1258-1262.

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