Effects of propofol on potassium currents in pulmonary artery smooth muscles of rats

Abstract

Abstract: AIM: To investigate the effects of propofol on potassium currents in pulmonary artery smooth muscle cells of normotensive and hypertensive rats. METHODS: The effects of propofol on potassium currents in smooth muscle cells derived from normotensive and hypertensive rats pulmonary arteries were observed by patch clamp technique (whole cell recording) after application of the drug in the bath. RESULTS: The potassium current-voltage curves (I-V curves) of smooth muscle cells derived from normotensive and pulmonary hypertensive rats pulmonary arteries were up-ward shifted by propofol (50, 100 μmol/L).Compared with control group, within 5 minutes after application of the drug, the current amplitude could increase to (121±11)%, (113±5)% (P<0.01) and (126±5)%, (122±8)% (P<0.01) of initial current amplitude respectively. CONCLUSION: The out-ward potassium currents in smooth muscle cells derived from normotensive and pulmonary hypertensive rats could be increased by propofol.This may be one of the mechanisms that propofol can decrease pulmonary vascular tension and dilate pulmonary vessel.

Key words: propofol, potassium channels, smooth muscle, blood vessel, pulmonary artery

CLC Number:

R965.2

JIANG Yu-ge, XU long-he, ZHANG Hong. Effects of propofol on potassium currents in pulmonary artery smooth muscles of rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(7): 756-759.

References

1 Park WK, Lynch C 3rd, Johns RA.Effects of propofol and thiopental in isolated rat aorta and pulmonary artery[J].Anesthesiology, 1992;77:956-963.
2 Rich GF, Roos CM, Anderson SM, et al.Direct effects of intravenous anesthetics on pulmonary vascular resistance in the isolated rat lung[J].Anesth Analg, 1994;78:961-966.
3 钱金桥, 杨炎华, 周定邦, 等.缺氧性肺血管收缩的细胞机制[J].医学综述, 2005;11:307-309.
4 Jeffery TK, Bryan-Lluka LJ, Wanstall JC.Specific uptake of 5-hydroxytryptamine is reduced in lungs from hypoxic pulmonary hypertensive rats[J].Eur J Pharmacol, 2000;396:137-140.
5 Cox RH, Petrou S.Ca(2+) influx inhibits voltage-dependent and augments Ca(2+)-dependent K(+) currents in arterial myocytes[J].Am J Physiol, 1999;277:C51-C63.
6 De La Cruz JP, Sedeno G, Carmona JA, et al.The in vitro effects of propofol on tissular oxidative stress in the rat [J]. Anesth Analg, 1998;87:1141-1146.
7 Mistry DK, Garland CJ.Nitric oxide (NO)-induced activation of large conductance Ca²⁺-dependent K⁺ channels (BK(Ca)) in smooth muscle cells isolated from the rat mesenteric artery [J].Br J Pharmacol, 1998;124:1131-1140.
8 YamazakiM, Nagakawa T, Hatakeyama N, et al.The effects of propofol on neural and endothelial control of in situ rat mesenteric vascular smooth muscle transmembrane potentials[J]. Anesth Analg, 2002;94:892-897.
9 Klockgether-Radke AP, Schulze H, Neumann P, et al.Activation of the K⁺ channel BK(Ca) is involved in the relaxing effect of propofol on coronary arteries[J].Eur J Anaesthesiol, 2004; 21:226-230.
10 沈红, 王虹, 杨玉.钾通道在肺动脉高压中的作用[J].国外医学(呼吸系统分册), 2003;23:180-182.
11 容俊芳, 张志刚, 高丽君, 等.静脉麻醉药对先心病合并肺动脉高压病人肺循环阻力的影响[J].中华麻醉学杂志, 2003;23:740-742.
12 Claeys MA, Gepts E, Camu F.Haemodynamic changes during anaesthesia induced and maintained with propofol[J].Br J Anaesth, 1988;60:3-9.

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