Effects of heparin on expression of leptin and transforming growth factor β1 in rat hepatic stellate cells

Abstract

Abstract: AIM: To explore the effects of heparin on the expression of leptin and transforming growth factor β₁ (TGF β₁) in rat hepatic stellate cells(HSCs). METHODS: HSCs were divided as follows:heparin group Ⅰ(10 μg/mL), Ⅱ(100 μg/mL), Ⅲ(1 000 μg/mL), and control group.The incubation liquid was extracted after cultured 48 h and frosted in-20 ℃.Leptin and TGF β₁ were detected by ELISA, the HSC proliferation was measured by MTT assay. RESULTS: The levels of leptin in heparin group Ⅱ, Ⅲ were significantly lower than that in control group(P<0.01).The level of TGF β₁ and the mean absorbance in heparin groups were significantly lower than that in control group (P<0.01). CONCLUSION: Heparin can decrease expression of leptin and TGF β₁ in HSCs and inhibit proliferation of HSCs.

Key words: hepatic stellate cell, heparin, leptin, transforming growth factor β₁, rat

CLC Number:

R965.2

CHEN Yi-ping, XU Zhi-wei, SHI Hai-fang, DI Jun-bo, CHEN Jun-ya, WANG Hong-jiao. Effects of heparin on expression of leptin and transforming growth factor β₁ in rat hepatic stellate cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(7): 760-762.

References

1 Xie X, Rivier AS, Zakrzewicz A, et al.Inhibition of selectinmediated cell adhesion and prevention of acute inflammation by nonanticoagulant sulfated saccharides.Studies with carboxyl-reduced and sulfated heparin and with trestatin a sulfate[J].J Biol Chem, 2000;275:34818-34825.
2 Otte C, Otte JM, Strodthoff D, et al.Expression of leptin and leptin receptor during the development of liver fibrosis and cirrhosis[J].Exp Clin Endocrinol Diabetes, 2004;112:10-17.
3 冷希圣, 翁山耕, 李涛, 等.大鼠肝星状细胞系的建立及其生物学特性的研究[J].解剖学报, 2003;34:269-274.
4 陈晓鹏, 刘颖斌, 史良会, 等.肝素对肝癌细胞乙酰肝素酶mRNA 表达和侵袭能力的影响[J].中国临床药理学与治疗学, 2006;11:810-813.
5 郝菁华, 朱菊人, 石军, 等.肝素对肝纤维化大鼠模型作用的疗效观察[J].胃肠病学和肝病学杂志, 2005;14:461-463, 467.
6 Friedman SL.Mechanisms of disease:Mechanisms of hepatic fibrosis and therapeutic implications[J].Nat Clin Pract Gastroenterol Hepatol, 2004;1:98-105.
7 Saxena NK, Titus MA, Ding X, et al.Leptin as a novel profibrogenic cytokine in hepatic stellate cells:mitogenesis and inhibition of apoptosis mediated by extracellular regulated kinase (Erk) and Akt phosphorylation[J].FASEB J, 2004;18:1612-1614.
8 Saxena NK, Ikeda K, Rockey DC, et al.Leptin in hepatic fibrosis: evidence for increase collagen production in stellate cells and lean littermates of ob ob mice[J].Hepatology, 2002;35: 762-771.
9 Leung JC, Chan LY, Tang SC, et al.Leptin induces TGF-beta synthesis through functional leptin receptor expressed by human peritoneal mesothelial cell[J].Kidney Int, 2006;69:2078-2086.
10 Jones E, Symes A.Leptin and TGF-beta synergistically regulate VIP cytokine response element transcription[J].Neuroreport, 2000;11:4049-4053.
11 Ikejima K, Honda H, Yoshikawa M, et al.Leptin augments inflammatory and profibrogenic responses in the murine liver induced by hepatotoxic chemicals[J].Hepatology, 2001;34:288-297.
12 Potter JJ, Rennie-Tankersley L, Mezey E.Leptin deficiency prevents the activation of the murine alpaha 2(I) collagen promoter by acetaldehyde[J].Arch Biochem Biophys, 2004;426: 73-77.
13 李文才, 张锦生, 黄光存, 等.肝素对大鼠肝星状细胞生长、细胞外基质和基质金属蛋白酶基因表达的影响[J].中华肝脏病杂志, 2000;8:200-202.

[1]	FU Xiaoyu, KONG Deguang, LI Juanjuan. Treatment progress of triple positive breast cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(8): 866-875.
[2]	CHEN Qun, ZHU Heping, YUAN Xiaolong. Analysis of 197 cases of pentoxifylline injection and exploring supervision mode of rational drug use in hospitals [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(8): 948-953.
[3]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[4]	XU Xiuhe, HE Xiaoli, ZHOU Jiashun, PAN Lizhu, ZHOU Zhuojun, LI Jiayue, WANG Caixia, YUAN Wei, ZHU Guiqi. Effects of scacia honey on serum uric acid level and renal injury in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 743-750.
[5]	WANG Lin, YU Jianguo, LI Xiao, LUAN Jiajie. Establish and implement a pre-prescription system for outpatient based on Six Sigma DMAIC model [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 780-787.
[6]	WANG Anjing, WANG Yaya, LIANG Xuan, YAN Yajie, SU Jing, LI Caidong. Research progress on mechanisms and therapeutic drugs of peroxisome proliferator-activated receptor in treatment of cholestatic liver disease [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 796-808.
[7]	WANG Donghui, JIANG Suwen, HU Airong, ZHU Bo, HE Zheyun, ZHANG Lukan, WANG Jialan, FAN Ying, LIN Ken . Mulberry exerts antioxidant stress effect in rats with nonalcoholic fatty liver disease [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 609-616.
[8]	LI Hongqin, CAO Limin, JIN Manyi, WANG Jianlong, CHAI Xiaoping, TIAN Miaomiao. Effects of different dosage forms of Qianzheng powder on facial nerve injury in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 617-623.
[9]	ZHANG Wei, LIU Lei, GUAN Shaoyang, SHENG Zhao, SUN Wenwen. Analysis of the diagnostic value of fine-needle aspiration cytology and BRAF and RAS gene mutation screening for thyroid cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 653-657.
[10]	WU Xiongzhi, WANG Xuan, XU Siqi, JU Xia, WANG Shengbin, CHEN Yongquan. A comparative investigation of the impact of oral midazolam solution and dexmedetomidine nasal spray on preoperative anxiety in pediatric patients [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 666-670.
[11]	QIAO Hongliang, DING Ning, DENG Kaihong, LIU Binbin, DONG Chuanjiang, CHEN Xiaobo, ZOU Lili. New progress in research on novel control strategies for methicillin-resistant staphylococcus aureus infection [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 676-687.
[12]	CHEN Cheng, DENG Yu, GONG Youlan, ZHANG Zhenming, DUAN Wulei, QING Jun, ZHANG Bo. Research progress of the classical TCM formula Huaihua powder [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(6): 697-704.
[13]	WANG Yan, ZHANG Jinhui, ZHAO Yongchen, LIU Hongxiang, LIU Yawei, MIAO Huanhuan, YANG Xincai. Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 508-513.
[14]	DANG Zilong, LIU Haiyang, ZHANG Yangfan, LIU Jinfeng, ZHANG Tianwei, LI Bin, GUO Hong, CHENG Wenyuan, XU Xiaohui, LI Yile, WEI Yuhui, WU Xin'an. Optimization strategy of antibiotic dosing regimen in intensive care unit patients with augmented renal clearance [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 561-571.
[15]	LIU Tong, XU Jiaming, WANG Jinhuo, YIN Lei, CHEN Yongquan, GUO Jianrong. Changes of anesthetic drug concentration in plasma during isolation of autologous blood with acute isovolumic hemodilution and its influence on anesthetic effect after reinfusion [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(4): 413-418.

Effects of heparin on expression of leptin and transforming growth factor β₁ in rat hepatic stellate cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments