Nimodipine inhibits ischemia-reperfusion triggered neurogenesis in adult rats

Abstract

Abstract: AIM: To observe the effects of Nimodipine on hippocampal dentate gyrus neurogenesis of the adult rat triggered by ischemia-reperfusion and investigate its molecular mechanism.METHODS: The cerebral ischemia-reperfusion models were established by four-vessel-blocked method.Nimodipine or the extracellular signal-regulated kinase inhibitor U0126 was administrated by intravenous injection or intraventricular injection, respectively before ischemia 20 min.The hippocampal dentate gyrus neurogenesis was detected by immunohistochemistry of 5-bromodeoxyuridine (Brdu).The expressions of ERK and p-ERK were detected by Western blotting.RESULTS: Nimodipine not only inhibited hippocampal dentate gyrus neurogenesis but also the expression of p-ERK in the area of dentate gyrus after ischemia-reperfusion.There was no significant difference in hippocampal dentate gyrus neurogenesis between the U0126 group and U0126 combined with Nimodipine group.CONCLUSION: Nimodipine inhibits hippocampal dentate gyrus neurogenesis, and it may be related with the down-regulation p-ERK after ischemia-reperfusion.

Key words: cerebral ischemia-reperfusion, neurogenesis, Nimodipine, extracellular signal-regulated kinase

CLC Number:

R966

TIAN He-ping, GU Bin, CHENG Feng, HAO Huai-yong, HU Wei-xin, LI Li-xin. Nimodipine inhibits ischemia-reperfusion triggered neurogenesis in adult rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(1): 32-36.

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