Effects of roxithromycin on NF-κB activity and bronchial hyperresponsiveness of asthma rats

Abstract

Abstract: AIM: To investigate the effect of roxithromycin on the NF-κB activity, the airway inflammation and bronchial hyperresponsiveness of asthma rats. METHODS: Thirty male adult Sprague-Dawley rats were randomly divided into the control group(Group C, n=10), asthma group (Group A, n=10) and roxith group(Group R, n=10).The asthma model was induced by ovalbumin and Al(OH) 3.The airway reactivity was detected and then rats in each group were sacrificed after 24 hours for the last provocation.The concentrations of IL-4, IL-5 and IFN-γin bronchoalveolar lavage fluid (BALF) were measured by ELISA, and the activity of NF-κB protein in bronchial epithelium was detected by immunohistochemistry.RESULTS: Compared with Group C, the airway reactivity in Group A was increased(P<0.01).Compared with Group A, the airway reactivity in Group R was decreased (P<0.05).Compared with Group A, the concentrations of IL-4, IL-5 in BALF in Group C were decreased (all P<0.01) and those in Group R were decreased (all P<0.01).The concentration of IFN-γ in BALF in Group R was significantly less than that in Group C (P<0.01) but was higher than that in Group A (P<0.01).The activity of NF-κB protein in bronchial epithelial in Group A was significantly higher than that in Group C (P<0.01) and that in Group R was less than that in Group A (P<0.01).There were significantly positive correlation between the activity of NF-κB p65 in bronchial epithelium and the concentrations of IL-4, IL-5 in BALF (r=0.856, P<0.01;r=0.912, P<0.01).There were significantly negative correlation between the concentrations of IL-4 and IL-5 in BALF and PC25Raw and PC15Cdyn (r=-0.713, P<0.01;r=-0.772, P<0.01, r=-0.738, P<0.01;r=-0.818, P<0.01, respectively).CONCLUSION: Roxithromycin can significantly inhibit the activity of NF-κB and retrieve the imbalance of Th1/Th2 cytokine in asthma.The airway reactivity of asthma rats is decreased by roxithromycin which may be related to the reduction of IL-4 and IL-5 expression

Key words: asthma, roxithromycin, NF-κB, bronchial hyperresponsiveness

CLC Number:

YAN Sun-shun, DAI Yuan-rong, WU Li-qin, XIA Xiao-dong. Effects of roxithromycin on NF-κB activity and bronchial hyperresponsiveness of asthma rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(7): 748-753.

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