Abstract: AIM: To observe the effects of receptor of advanced glycation endproducts(RAGE) , NF-κB double gene antisense RNA on the productions of TNF-αand IL-6 treated by advanced glycation endproducts (AGEs ).METHODS: The levels of TNF-αand IL-6 in the supernatants were measured by enzyme linked immunosorbent assay (ELISA) in ECV304 cells treated by AGEs.The RAGE, NF-κB single double gene antisense RNA were transfected into ECV304 cell.The expressions of RAGE and NF-κB were detected by flow cytometry and RT-PCR.In different clone cells, the effects of antisense RNA on the productions of TNF-α、IL-6 were determined by ELISA.RESULTS: AGEs, instead of human serum albumin (HSA) , stimulated ECV304 cell to produce TNF-α and IL-6 with a time-and dose-dependent manner.The RAGE, NF-κB single double gene antisense RNA were transfected into ECV304 cell.Induced by AGEs, the expressions of RAGE and NF-κB in double gene cotransfected cell were inhibited by (62.2 ±8.7) % and (37.2 ±7.1) %, respectively.Induced by AGEs, the amount of TNF-α、IL-6 in the medium were lower in single gene transfected cells ECV-asRAGE, ECV-asP65 than ECVVector(P <0.05).The amount of TNF-α、IL-6 in the double gene transfected cells ECV-asRAGE-asP65 were lower than ECV-Vector and ECV-asRAGE, ECV-asP65(P<0.05).CONCLUSION: The production of TNF-α and IL-6 is increased in ECV304 cell treated by AGEs.RAGE, NF-κB double gene antisense RNA can inhibited the production of inflammatory factor treated by AGEs.

Key words: antisense RNA, advanced glycation endproducts, receptor, nuclear factor-κB, gene transfection, inflammatory factor