Calcium caused calcium release causes a vascular smooth muscle cell line A10 cells apoptosis

Abstract

Abstract: AIM: To investigate the apoptosis of a vascular smooth muscle cell line A10 caused by mild K+depolarization.METHODS: Apoptosis was evaluated by nuclear staining, DNA fragmentation gel electrophoresis and propidium iodide-stained flow cytometry.Mitochondrial transmembrane potential (Δψm) was measured by flow cytometry.RESULTS: K+depolarization caused dose correlated A10 cells apoptosis; nifedipine, BAPTA AM, ryanodine inhibited the cytotoxic effect of K+completely.The combination use of nifedipine and cyclosporin A made it clear that mitochondria was involved in the apoptosis of A10 cells, and Δψm measurement further confirmed this speculation; A10 apoptosis caused by K+depolarization was not influenced by heparin or Zn²⁺, a effectivecapacitative calcium entry(CCE) blocker.CONCLUSION: Ca²⁺entry through voltage-dependent ca channels increases intracytoplasm Ca²⁺, then triggers furtherCa²⁺release from endoplasmic reticulum via ryanodine receptor, and the microdomains of elevated intracytoplasmCa²⁺are sensed by adjacent mitochondria, which ultimately lead to cell apoptosis.

Key words: vascular smooth muscle cell, apoptosis, calcium release, ryanodine, mitochondria

CLC Number:

R969

WU Yu-lin, MA Bing-liang , DAI Xiao-ming, CHEN Lin-lin. Calcium caused calcium release causes a vascular smooth muscle cell line A10 cells apoptosis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(7): 789-796.

References

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