Effect of Bacille Calmette Guerin inoculation with or without IFN-γ/Vit A on immunologic status of infant with bronchiolitis

Abstract

Abstract: AIM: To discuss the effects of Bacille Calmette Guerin (BCG) inoculation with or without supplement of vitamin A/IFN-γon tubercle bacillus protein purified derivative (PPD) skin test, wheezing episode and in vitro the cytokine levels of peripheral blood mononuclear cells (PBMC) in infants with acute bronchiolitis.METHODS: 44 acute bronchiolitis infants (ABI) were treated with BCG alone or BCG combined with Vitamin A/IFN-γ.19 ABI treated without BCG as control.PPD skin test, in vitro production of IL-10 、IL-12 、IL-18 、IL-4 and IFN-γby supernatant of PBMC of bronchiolitis measured by ELISA, and expression of lymphocyte activation gene-3 (LAG-3) mRNA from PBMC of bronchiolitis measured by semi-quantitative RT-PCR were observed before and 3 months after BCG inoculation.Follow upfor average 12 months was to observe thewheezing episodes.RESULTS: The diameters of PPD skin test in ABI after BCG inoculation combined with IFN-γ/VitA were larger than those of ABI before inoculation and control group.The frequencies and persistence of wheezing were lower in interfered infants with acute bronchiolitis either by BCG alone or BCG combined with IFN-γ/VitA than those of control groupby 1 year.IL-12 、IL-18 and IFN-γactivities in ABI treated with BCG or BCG combined with IFN-γ/Vitamin A in vitro were higher than those of pre-treatment bronchiolitis infants;the level of IL-4 was lower;the level of IL-10 was a little bit lower, and expression of LAG-3 mRNA was stronger.Frequency of wheezing episode in 1 year in acute bronchiolitis infants with BCG or BCG combined with IFN-γ/Vitamin A was lower than that of control group.There were no difference between ABIwith BCG or BCG combined with IFN-γ/Vitamin A.Clinical manifestation and the level of cytokines do not show superiority in BCG with IFN-γ/Vitamin A.CONCLUSION: BCG inoculation is able to enhance PPD skin test response,promote Th1 reactivity, and prevent from recurrent wheezing after acute bronchiolitis.The level of IL-10 was degraded lightly after BCG intervention, presumably concerning with regular T cells(CD4 +CD25 +Tr) induced by BCG inoculation.

Key words: bronchiolitis, Bacille Calmette Guerin, PPD skin test, TH1/TH2, cytokine

CLC Number:

R965.2
R966

LIU Ya-li, YANG Xi-qiang<. Effect of Bacille Calmette Guerin inoculation with or without IFN-γ/Vit A on immunologic status of infant with bronchiolitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(9): 1030-1034.

References

1 Martin MA.Respiratory syncytial virus infection and asthma[J].Allergol Immunopathol, 2001;29:140-6
2 Kim CK, Kim SW, Park CS, Kim BI, Kang H, Koh YY.Bronchoalveolar lavage cytokine profiles in acute asthma and acute bronchiolitis[J].J Allergy Clin Immunol, 2003;112:64-71
3 Annunziato F, Manetti R, Tomasevic I, Guidizi MG, Biagiotti R, Gianno V, et al.Expression and release of LAG-3-encoded protein by human CD4+T cells are associated with IFN-gamma production[J].FASEB J, 1996;10:769-76
4 Trefny P, Stricker T, Baerlocher C, Sennhauser FH.Family history of atopy and clinicl course of RSV infection in ambulatory and hospitalized infants[J].Pediatr Pulmonol, 2000;30:302-6
5 Shirakawa T, Enomoto T, Sasaki S, Taniguchi K, Motonaga M, Akahori W, et al.The inverse association between tuberculin responses and atopic disorder[J].Science, 1997;275:77-9
6 Koh YI, Choi IS, Kim WY.BCG infection in allergen-presensitized rats suppresses Th2 immune response and prevents the development of allergic asthmatic reaction[J].J Clin Immunol,2001;21:51-9
7 Horner AA, Van Uden JH, Zubeldia JM.DNA-based immunotherapeutics for the treatment of allergic disease[J].Immunol Rev, 2001;170:102-18
8 Choi IS, Koh YI.Therapeutic effects of BCG vaccination in adult asthmatic patients:a randomized, controlled trial[J].Ann Allergy Asthma Immunol, 2002;88:584-91
9 Choi IS, Koh YI.Effects of BCG revaccination on asthma.Al-lergy , 2003;58:1114-6
10 Choi IS, Koh YI.Therapeutic effects of BCG vaccination in adult asthmatic patients:arandomized controlled trial[J].Ann Allergy Asthma Immunol, 2002;88:584-91
11 Shirtcliffe PM, Easthope SE, ChengS, Weatherall M, Tan PL,LeGros G, et al.The effect of delipidated deglycolipidated (DDMV) and heat -killed Mycobacterium vaccae in asthma [J].Am J Respir Crit Care Med, 2001;163:1410-4
12 Wong GW, Hui DS, Tam CM, Chan HH, Fok TF, Chan-Yeung M, et al.Asthma, atopy and tuberculin responses in Chinese schoolchildren in Hong Kong[J].Thorax, 2001;56:770-3
13 刘恩梅, 罗嘉慧, 李秋, 杨锡强.比较卡介苗及其成分对脐血单核细胞转化树突状细胞成熟影响[J].临床儿科杂志, 2002;20:589-91
14 Youngil I, Koh Inseon S, Choi Je-Jung L.Effects of Cytokine Milieu Secreted by BCG-treated Dendritic Cells on Allergen-Specific Th Immune Response[J].J Korean Med Sci, 2004;19:640-6
15 Jeffrey AB, Abul KA.Natural versus adaptive regulatory T cells[J].Nat Rev Immunol, 2003;3:253-7
16 Taylor A, Verhagen J, Akdis CA, AkdisM.T regulatory cells in allergy and health:a question of allergen specificity and balance[J].Int Arch Allergy Immunol, 2004;135:73-82
17 Akbari O, Freeman GJ, Meyer EH.Antigen-specific regulatory T cells developvia the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity[J].NatMed, 2002;8:1024-32
18 Zuany-Amorim C, Sawicka E, Manlius C, Le Moine A, Brunet LR, Kemeny DM, et al.Suppression of airway eosinophilia by killedMycobacterium vaccae-induced allergen-specific regulatory T-cells[J].Nat Med, 2002;8:625-9

[1]	MA Yitong, MA Jianhong, GAO Yating, LIU Chang. Pathogenic mechanism and research progress of adipocytokines in endometrial cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1315-1320.
[2]	HUANG Hao, JIA Hong, WANG Xiaoshuang, ZHANG Lu, JIANG Hua. Effects of co-culture of dendritic cells loaded with MAGE-A3 antigen and cytokine-induced killer cells on tumor stem cells and malignant progression of endometrial cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(1): 42-50.
[3]	GAO Jiarong, SHI Miaomiao, CHEN Hao, JIANG Hui, QIN Xiujuan. Effect of Qiteng Xiaozhuo granule mediating miR-339-5p on inflammatory indexes in rats with chronic glomerulonephritis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(4): 457-465.
[4]	HE Xiujuan, MA Huike, LIU Qingwu, CHEN Jia, LIN Yan, LI Ping. Research progress of cytokines in wound healing [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(5): 579-585.
[5]	WU Jiayuan, ZHENG Li, MO Juanfen, GUO Li, CAO Chenxi, BAO Yi. Regulation of N-acetylcysteine on peripheral circulatory and hepatic myeloid-derived suppressor cells in non-alcoholic fatty liver disease mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(9): 989-996.
[6]	LI Min, WANG Shanshan, LIU Zhonghe, JIANG Deguo. Expression and mechanism of p38MAPK signaling pathway in chronic pulpitis rats with pain [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 644-649.
[7]	BU Zhangyu, WU Liming, YU Xiaohong, LIU Jue, DENG Lin. Investigation of curative effect and mechanism of Chaihu Jiedu Decoction on condyloma acuminatum [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 681-686.
[8]	ZHAI Shuo，LI Na，CHEN Beining，KOU Junping，XING Weifan，ZHANG Pinghu. Prospective of a natural remedy, silymarin from milk thistle, for treating chronic hepatitis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(5): 573-579.
[9]	HU Yuping, ZHOU Haiyun, HUANG Qiaoling, YAO Yimin. Effect of paeonol on the expression of cytokines production and maturation of DCs co-stimulated by FSL-1 and IL-4 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(1): 14-19.
[10]	MAO Qingdong. Efficacy and safety of recombinant human interferon alfa2b oxygen inbreathe for bronchiolitis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 927-931.
[11]	HUANG Lijia, HUANG Yang, KONG Jinsong, ZHENG Xin, GONG Xiaokang, RUAN Jianwei, WANG Haibao. Inhibition of articular cartilage degeneration by atorvastatin calcium via STAT1-caspase-3 signaling axis in rats with knee osteoarthritis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(7): 743-748.
[12]	WANG Xianhai, LI Zhenbin, LI Xiaomei, ZHANG Jianguo, SHI Xinmin, XU Haiyan, FENG Minlu, SHI Mengqiong . Study of Tongjing dingyunwan blocking NF-κB activation on the MCP-1/CCR2 signaling pathway and expressions of TNF-α, IL-1β, IL-6 in the cervical spondylosis rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 370-376.
[13]	LI Fang, ZHENG Liupu, WENG Jianlong, GUO Tao, SONG Xingwei, ZHENG Rongyuan. Therapeutic time window of effectiveness for idazoxan ameliorating brain damage of rats with experimental autoimmune encephalomyelitis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 1-7.
[14]	SONG Meiqing. Effect of metformin on bone mineral density, insulin resistance and cytokines in type 2 diabetes mellitus patients with primary hypertension [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(6): 694-698.
[15]	MAO Hongxia, JIN Lingqing, LIU Shuangchun. Effect of glucosamine sulfate combined with Shenmai injection on knee osteoarthritis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(5): 560-564.