Abstract: AIM: To study the correlation between pharmacodynemics and pharmacokenitics characteristics of baicalin using serum pharamoclogical test in vitro.METHODS: After administration of baicalin in rats, the drug-contained serum was obtained at different time and each sample was divided into two portions.One portion was respectively added into the cultured PC12 cells,which had been treated with H2O2, and the viability was determined by MTT colormetric microassay.The otherwas used to determine the concentration of baicalin in each serum sample.Then the relationshiPamong concentration,time and effect could be established.Furthermore, the relationshiPof concentration-effect of bacalin solution was also investigated in vitro.RESULTS: Exact antioxidant effects of baicalin solution and drug-contained serum could be shown in vitro test.The curves of time-effect and time-concentration were both peaked at 15 min after oral administration.CONCLUSION: The protection rate of the baicalin-contained serum had a positive correlation with the AUC of baicalin in serum.

Key words: baicalin, PC12 cells, serum pharmacology, pharmacokenetics-pharmacodynamics model