Abstract: AIM: To research the inhibitory effect of antihypercoagulability state enzyme (AHCSE) of snake venom in different dosages in mice with Hepatocarcinoma.METHODS: Hepatic cancer H22 model in mice was performed through subcutaneous inoculation of Right-Sidedness Axilla of BALB/C mice.Different dosages of AHCSE (1, 2, and 4 mg·kg^-1) were injected via abdominal cavity everyday for 10 days.When the tumor grew to 10 mm³, the weight, volume, percent inhibition of tumor, spleen index and the number and the component of white blood cells were observed.RESULTS: The middle and high dosages of AHCSE had obvious inhibition in hepatic cancer.It showed a significant dose-dependent relation in the effects of AHCSE on neoplastic weight, neoplastic volume and splenic index.There was a linear regression between the number of leucocytes and the splenic index.CONCLUSION: AHCSE of 2-4 mg·kg^-1 has significant inhibitory effect on tumor, and shows dose-related effect in mice with Hepatocarcinoma.Its mechanism is presumably associated with activating immune system.

Key words: antihypercoagulability state enzyme of snake venom, hepatic cancer, antitumor