Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2005, Vol. 10 ›› Issue (3): 265-269.

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Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia

LIU Hong-liang, DAI Ti-jun¹, YAO Shang-long

Department of Anesthesiology, Union Hospital Tongji Medical College, Huazhong Technology University, Wuhan 430030, Hubei, China;
¹Department of Anesthesia Pharmacology, Xuzhou Medical College, Xuzhou 221002, Jiangsu, China

Received:2004-10-30 Revised:2004-12-13 Online:2005-03-26 Published:2020-11-18
Contact: LIU Hong-liang, male, medical doctor, engaged in the study of mechanisms and effects of general anesthesia.Tel:027-62535517 E-mail:liuhl75@163.com
About author:DAI Ti-jun, male, professor, advisor of master, engaged in anesthesia pharmacology. YAO Shang-long, male, professor, advi sor of doctor, engaged in the study of mechnisms and effect s of general anesthesia
Supported by:
Supported by National Natural S cience Fund of China (№39970715)

Abstract

Abstract: AIM: To investigate the dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases using thiopental and propofol anesthesia.METHODS: Thiopental of 30 mg°kg^-1 or propofol of 100 mg°kg^-1 was administered respectively at different anesthetic phases (induction, anesthesia, restoration, and awake), the activities of NOS and ATPase and NO production in cortex and brain stem were measured.Saline 10 ml°kg^-1 was injected as the control group.RESULTS: Ca²⁺-ATPase and Na⁺, K⁺-ATPase activities in the cortex and brain stem were significantly decreased after administration of thiopental and propofol, especially at induction, anesthesia, or even restoration phase of thiopental group (P<0.05, P<0.01) and at anesthesia phase of propofol group (P<0.05).NOS activities and NO production decreased from induction to restoration phase with thiopental and propofol anesthesia (P<0.01).The parameters were returned near to the normal at awaken phase.CONCLUSION: Activities of ATPases and NOS and the production of NO may mediate the anesthesia effects of thiopental and propofol in the rat cortex and brain stem.

Key words: ATPase, nitric oxide synthase, nitricoxide, thiopental, propofol

CLC Number:

R966
R971.2

LIU Hong-liang, DAI Ti-jun, YAO Shang-long. Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(3): 265-269.

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Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia

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