Effects of iptakalim hydrochloride on H2O2 induced cytotoxicity in pheochromocytoma(PC12) cells

Abstract

Abstract: AIM: To investigate the protective effects and mechanisms of iptakalim hydrochloride(Ipt) on H₂O₂ induced neurotoxity. METHODS: Neurotoxity injury was induced by H₂O₂ in PC12 cells.The cell viability was tested by MTT assay. The glutamate released from PC12 cells was measured by HPLC combined with fluorescent detector analysis.Changes in the intracellular free Ca²⁺ concentration ([Ca²⁺]_i) were determined in fluo-3 AM loaded PC12 cells.RESULTS: Ipt (1, 10 and 100μmol°L^-1) markedly mitigated H₂O₂ -induced neurotoxi-ty, 10μmol°L^-1 Ipt inhibited the release of glutamate and the increase of ([Ca²⁺]_i) induced by H₂O₂. The pro-tective effectswas incompletely blocked by 5-HDwhich is a mitochondrial K ATP channels antagnist. CONCLUSION: Ipt provides neuroprotective effects on H₂O₂ in-duced cytoxixity in cultured PC12 cells and the protective effects may be partially related with mitochondrial KATP channels.

Key words: iptakalim hydrochloride, ATP-sensitive potassium channels, PC12 cell, oxidative stress, gluta-mate, intracellular free Ca²⁺

CLC Number:

R971.1

CHAI Yi, DING Jian-hua, LI Hao, HU Gang. Effects of iptakalim hydrochloride on H₂O₂ induced cytotoxicity in pheochromocytoma(PC12) cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(4): 377-381.

References

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Effects of iptakalim hydrochloride on H₂O₂ induced cytotoxicity in pheochromocytoma(PC12) cells

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