Effects of diazoxide on membrane potential and respiration function of rat cardiac mitochondria

Abstract

Abstract: AIM: To study the effects of selective mitoKATP opener diazoxide on the isolated mitochondria from rat hearts and to discuss its cardioprotective mechanism. METHODS: Using Rhodamine123 (Rh123)as fluorescent prober to measure mitochondrial membrane potential and to record mitochondrial respiratory parameters with a Clark electrode, the effect of diazoxide on mitochondrial membrane potential and mitochondrial respiration were investigated. RESULTS: 50 μmol·L^-1 diazoxide could induce mitochondrial membrane depolarization and this effect was abolished by application of a KATP channel blocker.It could decrease R3 and R4 while oxidizing FAD-dependent substrates (succinate)but did not affect respiratory control ratio (RCR). CONCLUSION: Modulation by diazoxide on functions of rat cardiac mitochondria is associated with its anti-ischemic efficacy.

Key words: diazoxide, mitochondria, Clark electrode, mitochondrial membrane potential

CLC Number:

R965

ZENG Yuan, LONG Chao-Liang, MU Shao-Feng, LI Yan-Fang, WANG Hai. Effects of diazoxide on membrane potential and respiration function of rat cardiac mitochondria[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(5): 490-493.

References

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