Abstract: AIM: To evaluate association of genotype and phenotype of CYP2D6 and the application of oligonu-cleotide microarray hybridization genetic testing in CYP2D6 multiple alleles analyses.METHODS: Two hundred forty-two healthy volunteers were recruited, and a 60 mg oral dose of dextromethorphan (DXT) was ad-ministered to each for assessment of the DXT metabolic ratio [MR].A 20 ml blood sample was also collected for DNA isolation and testing.CYP2D6 alleles *2——— * 11;*17 and multiple CYP2D6 gene copies were tested by allele-specific PCRand/or the affymetrix CYP450 gene chip assay.Five of the CYP2D6 alleles (*3, *4, * 6, *7, and *9) were evaluated by both PCR and the CYP450 gene chip assay.RESULTS: The CYP2D6 genotype was more informative and reflective in CYP2D6 enzyme expression than a phenotype.Genetic tests for the CYP2D6 *3, *4, *6, *7 and *9 alleles showed a high degree of concordance between the CYP450 gene chip and AS-PCR methods.CONCLUSION: Oligonu-cleotide microarray hybridization is a promising and reli-able approach for detecting multiple alleles at gene loci.

Key words: CYP2D6, phenotype, genotype, PCR, oligonucleotide microarray hybridization