Effects of fenofibrate on proliferation and apoptosis induced by lysophos-phatidylcholine in cultured human umbilical vein endothelial cells

Abstract

Abstract: AIM: To investigate the effect of fenofi-brate on proliferation and apoptosis induced by lysophos-phatidylcholine(LPC) in cultured human umbilical vein endothelial cells (HUVECs) and itsmechanism.METHODS: HUVECs were cultured in vitro.The study was designated to 5 groups:normal control;group LPC;low-concentration fenofibrate(10 μmol·L^-1) group;middle-concentration fenofibrate (50μmol·L^-1) group;high-con-centration fenofibrate(100 μmol·L^-1) group.Proliferation and apoptosis of HUVECs were evaluated by MTT assay, flow cytometry (FCM) and fluorescence microscopy.The expression of XIAP proteinwas examined by immunohisto-chemistry method.RESULTS: Compared with control group, LPC could inhibit the growth and induce apoptosis of HUVECs, and decrease the expression of XIAP protein in HUVECs.Fenofibrate could increase the growth and decrease the apoptosis of HUVECs, and enhance the ex-pression of XIAP protein in HUVECs.CONCLUSION: Fenofibrate can improve proliferation and apoptosis in-duced by lysophosphatidylcholine in HUVECs by enhanc-ing expression of XIAP protein.

Key words: fenofibrate, human umbilical vein endo-thelial cells, proliferation, apoptosis, XIAP

CLC Number:

SUN Guo-ju, XIE Xiu-mei, XING Ying, YANG Tian-lun, YU Guo-long. Effects of fenofibrate on proliferation and apoptosis induced by lysophos-phatidylcholine in cultured human umbilical vein endothelial cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(5): 535-539.

References

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