Artesunate enhance the ability of peritoneal macrophages endocytosing LPS and Escherichia coli and its mechnisms

Abstract

Abstract: AIM: To investigate the effect of artesunate (AS) on peritoneal macrophages endocytosing lipopolysaccharide/endotoxin (LPS) and phagocytosing Escherichia coli.METHODS: Effects of various concentrations of monodansylcadaverine (MDC) and chloroquine(CQ) on the viability of peritoneal macrophages were observed by MTT test. Effects of artesunate, monodansylcadaverine and chloroquine on LPS internalization of peritoneal macrophages were detected by confocal laser scaning microscopy. The effect of artesunate on Escherichia coli internalization of peritoneal macrophages was examined by confocal laser scaning microscopy and bacterial clone forming counting test. The effect of artesunate on class A scavenger receptor (SR-A) mRNA expression was measured using RT-PCR method.RESULTS: 25 μg /mL of MDC and 20 μg /mL of chloroquine had no effect on the viability of peritoneal macrophages. Artesunate could increase LPS internalization of peritoneal macrophages, while MDC and chloroquine could inhibit LPS but artesunate could still increase LPS internalization of macrophages treated with MDC and chloroquine. Artesunate could increase Escherichia coli internalization, and artesunate could up-regulate SR-A mRNA expression of peritoneal macrophages treated with or without LPS.CONCLUSION: Artesunate could enhance the ability of the peritoneal macrophages to internalize LPS and Escherichia coli, which was associated with up-regulation of SR-A mRNA expression of the peritoneal macrophages.

Key words: Artesunate, Internalization, Lipopolysaccharide, Escherichia coli, Scavenger receptor

CLC Number:

R965.2

YU Meng-chen, PAN Xi-chun, LI Xiao-li, LI Jun, XIAO Kang-kang, LI Bin, ZHOU Hong. Artesunate enhance the ability of peritoneal macrophages endocytosing LPS and Escherichia coli and its mechnisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(1): 40-46.

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