Mechanisms of lumiracoxib on apoptosis in human lung cancer cells

Abstract

Abstract: AIM: To investigate the possible mechanism of Lumiracoxib (LUM)on the apoptosis of lung cancer cells.METHODS: Cells growing to confluence were treated with IL-1β (1 μg/L) prior to stimulation with LUM(0,15,30,60 μmol/L)for 24 hours. Western blot analysis was used to examine the level of ERK2, p-ERK and Bcl-2 protein in human NSCLC cell lines. Western blot analysis was used to examine the expression of Bcl-2 and Bax in A549 and NCI-H460 cells after exposed to various concentrations of LUM.RESULTS: LUM inhibited the up-regulated ERK2 and p-ERK as well as the down-regulated Bcl-2 expression in A549 and NCI-H460 cells. Additionally, LUM decreased the expression of Bcl-2 protein. And it increased the expression of Bax in A549 cells dependently on its doses. However, it had no effect on NCI-H460 cells. The ratio of Bcl-2/Bax decreased correspondingly.CONCLUSION: The mechanisms of the apoptotic effect of Lumiracoxib may be related to regulating Bcl-2 via ERK signal transduction pathway in human NSCLC cell lines.

Key words: Lumiracoxib, Lung cancer, Apoptosis, Mechanism

CLC Number:

HAO Ji-qing, SUN Gen-yun, SHEN Yu-xian. Mechanisms of lumiracoxib on apoptosis in human lung cancer cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(1): 47-51.

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