Effect of oligomeric grape seed proanthocyanidins on prevention isoproterenol induced cardiomyocytes hypertrophy

Abstract

Abstract: AIM: To investigate the effect of oligomeric grape seed proanthocyanidins (GSP) on neonatal cardiomyocytes hypertrophy induced by isoproterenol (Iso).METHODS: Hypertrophy in cultured neonatal rat cardiac myocytes was induced by Iso. Total protein content was measured by BCA method; reactive oxygen species (ROS) were detected by flow cytometry; colorimetric method was employed to measured MDA content and SOD antivity.RESULTS:1×10^-5 mol/L Iso can activate β-AR completely and induce cardiomyocytes hypertrophy markedly. GSP therapy potently inhibited the increase of protein content, ROS generation and MDA content, inhibited the decrease of SOD activity with a dose-dependent manner.CONCLUSION: GSP possess protective effect against Iso induced cardiomyocytes hypertrophy, this may be related to reducing the ROS generation and oxidative stress level, at least in part.

Key words: Oligomeric grape seed proanthocyanidins, Isoproterenol, Cardiomyocytes hypertrophy, Reactive oxygen species, Malondialdehyde (MDA), Superoxide dismutase (SOD)

CLC Number:

R965.2

GAO Shan, HUANG Chun-xia, HUANG Ling-ling, YU Ting-ting, WANG Xing-hui, ZHANG Ye. Effect of oligomeric grape seed proanthocyanidins on prevention isoproterenol induced cardiomyocytes hypertrophy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(5): 497-501.

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