Development of pyrosequencing method for detection of SLCO1B1 polymorphisms

Abstract

Abstract: AIM: To establish a pyrosequencing based method for detection SLCO1B1 A388G and T521C polymorphisms and to determine the frequency of these polymorphisms in healthy Chinese.METHODS: After preparation of gDNA from blood of 300 subjects, the target fragments were amplified by PCR, polymorphisms were detected on PyroMark ID by pyrosequencing technology. The reliability of pyrosequencing methods were validated by repeat tests and Sanger sequencing.RESULTS:We established a new pyrosequencing method to detect the SLCO1B1 A388G and T521C polymorphisms polymorphisms in healthy Chinese. The detection rate and repetition rate were both 100%. The frequencies of 388A, 388G, 521T and 521C alleles were 28%,72%,89.5% and 10.5%, respectively. Genotype frequencies match the Hardy-Weinberg equilibrium.CONCLUSION: These pyrosequencing assays to detect SLCO1B1 polymorphisms are proved to be a rapid, accurate and high-throughput alternative to conventional methods, and it can be a preferred option in research and clinical application.

CLC Number:

R968

WAN Zi-rui, XIE Hai-tang, GUO Dong, HU Dong-li, WANG Li-ping, WANG Guo. Development of pyrosequencing method for detection of SLCO1B1 polymorphisms[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(5): 543-548.

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