Effects of sesamin on aortic relaxation in renal hypertensive-hyperlipidemia rats

Abstract

Abstract: AIM: To investigate the possible in vivo protective effects of sesamin on aortic relaxation in renal hypertensive-hyperlipidemia rats (RHHR).METHODS: RHHR were orally administrated with sesamin at a dose of 100, 33 and 10 mg·kg^-1·d^-1, respectively. After 8 weeks of treatment, the vasorelaxation response to acetylcholine (ACh) and sodium nitroprusside (SNP) was measured in aortic rings. Furthermore, the aortic response to ACh after being incubated with N-nitro-L-arginine-methyl-ester and NO bioactivity were determined.RESULTS: Compared with the sham-operated rats, ACh- and SNP- induced aortic vasorelaxation and NO bioactivity were obviously decreased in RHHR. These abnormalities were essentially reversed by treatment with sesamin for 8 weeks.CONCLUSION: All these results suggest that chronic treatment with sesamin reduces hypertension through improvement of impaired endothelium-dependent and -independent vasorelaxation in RHHR.

Key words: Sesamin, Renal hypertensive-hyperlipidemia rat, Endothelium-dependent relaxation, Endothelium-independent relaxation

CLC Number:

R965.1

KONG Xiang, YANG Jie-ren, GUO Li-qun, ZHOU Yong. Effects of sesamin on aortic relaxation in renal hypertensive-hyperlipidemia rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(4): 366-370.

References

[1] Daisuke N, Chie I, Matsumura Y, et al. Effects of sesamin on aortic oxidative stress and endothelial dysfunction in Deoxycorticosterone cetate-salt hypertensive rats [J]. Biol Pharm Bull, 2003, 26(12): 1701-1705.
[2] Kong X, Yang JR, Guo LQ, et al. Sesamin improves endothelial dysfunction in renovascular hypertensive rats fed with a high-fat, high-sucrose diet [J]. Eur J Pharmacol, 2009, 620(1/2/3):84-89.
[3] 孔祥, 杨解人. 芝麻素对肾性高血压大鼠主动脉收缩功能、NO和ET含量的影响 [J]. 中国实验方剂学杂志, 2006, 12(4): 47-49.
[4] 周勇, 杨解人. 芝麻素对代谢综合征大鼠血糖、血脂及主动脉血管细胞黏附分子-1蛋白表达的影响 [J]. 中国临床药理学与治疗学, 2008, 13(2): 45-49.
[5] Miyawaki T, Aono H, Toyoda-Ono Y, et al. Antihypertensive effects of sesamin in humans [J]. J Nutr Sci Vitaminol (Tokyo), 2009, 55(1): 87-91.
[6] Daisuke N, Chol-Jun K, Matsumura Y, et al. Sesamin metabolites induce an endothelial nitric oxide-dependent vasorelaxation through their antioxidative property-independent mechanisms: possible involvement of the metabolites in the antihypertensive effect of sesamin [J]. J Pharmacol Exp Ther, 2006, 318: 328-335.
[7] 孔祥, 杨解人, 郭莉群, 等. 单纯肾性和复合型肾性高血压大鼠主动脉功能的对比研究 [J]. 中国药理学通报, 2009, 25(2): 252-255.
[8] 张东, 武海军, 陈士萍, 等.大鼠实验性高脂血症五种造模方法的比较 [J]. 中国药理学通报, 2007, 23(9): 1254 - 1256.
[9] Kong X, Zhang DY, Wu HB, et al. Losartan and pioglitazone ameliorate nephropathy in experimental metabolic syndrome rats [J]. Biol Pharm Bull, 2011, 34(5): 693-699.
[10] 郭莉群, 杨解人, 孔祥. 芝麻素对高脂血症大鼠血脂及脂肪肝形成的影响[J]. 中国实验方剂学杂志, 2009, 15(2): 55-58.
[11] Cappelli-Bigazzi S, Rubattu C, Battaglia R, et al. Effects of high-cholesterol and atherogenic diets on vascular relaxation in spontaneously hypertensive rats [J]. Am J Physiol Heart Circ Physiol, 1997, 273(2): 647- 654.