Effects of 5-Aza-2'-deoxycytidine on the endometrium receptivity from endometriosis through modulation  HOXA10 gene

Abstract

Abstract: AIM: To study the effect of 5-aza-2'-deoxycytidine (AZA) on the endometrium from endometriosis.METHODS: Primary culture endometrial epithelia cells from endometriosis were treated with AZA for 24 h. Bisulfite sequencing were performed to detect difference in methylation patterns. The expression levels of HOXA10 were measured with Real-time reverse transcriptase (RT-PCR) and Western Blot.RESULTS: After treatment with AZA for 24 h,the promoter region of the HOXA10 gene exhibited a demethylation status,and the expression of HOXA10 was increased.CONCLUSION: The AZA could reverse methylation of HOXA10 gene to regulate the expression of HOXA10 gene, then inducing the endometrial receptivity in endometriosis.

Key words: 5-Aza-2'-deoxycytidine, Endometriosis, Endometrial receptivity, HOXA10, Methylation

CLC Number:

R965.2

WANG Li, WU Yu, LU Xiang, WANG Min, SUN Jian, GAO Xiao-hong, SUN Ying, LI Xin-yan. Effects of 5-Aza-2'-deoxycytidine on the endometrium receptivity from endometriosis through modulation HOXA10 gene[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(4): 361-365.

References

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Effects of 5-Aza-2'-deoxycytidine on the endometrium receptivity from endometriosis through modulation HOXA10 gene

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