Experimental study of Sijunzi Decoction and its components on the growth inhibiting of the Side Population(SP) Cells in MKN-28,SGC-7901 and BGC-823

Abstract

Abstract: AIM: To investigate the effect of Sijunzi decoction and its separate components on the growth and proliferation of the side population(SP) cells in human carcinoma cell lines MKN-28,SGC-7901 and BGC-823 in vitro.METHODS: Side population cells of three human gastric cancer cell lines with different differentiation grades named MKN-28,SGC-7901 and BGC-823,were analyzed and isolated by flow cytometryafter being stained by fluorochrome Hoechst33342 and PI, then characterized by cell growth curve (MTT) and colony formation assay. The effects of Sijunzi decoction and its separate components on the growth and proliferation of the side population(SP) cells were detected by MTT assay.RESULTS: SP cells were detected in all of the three gastric cancer cell lines with a proportion of 3.40%, 2.00%, 1.07%. Cell growth curves indicated that SP cells have stronger proliferation ability (P<0.05) compared with non-SP cells. Colony formation assay showed that the colony forming efficiency of the SP cells was higher than the total cells without sorting and the non-SP cells (P<0.01). Comparing with controls, the growth and proliferation of the SP cells were significantly inhibited by Sijunzi decoction and its separate components, and the inhibiting rates revealed in a mediated serumdose-dependent manner.CONCLUSION: Sijunzi decoction and its separate components have the growth-inhibitive effect on SP cells sorting from human carcinoma cell lines MKN-28,SGC-7901 and BGC-823 in vitro.

Key words: Sijunzi decoction, Medicated serum, Side population, MKN-28, SGC-7901, BGC-823, Growth inhibiting

CLC Number:

R965.1

JIA Jian-guang, MA Li, LI Jing, JIN Xin, ZHANG Li-gong, LIU Jian-wen, QIAN Jun. Experimental study of Sijunzi Decoction and its components on the growth inhibiting of the Side Population(SP) Cells in MKN-28,SGC-7901 and BGC-823[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(5): 513-518.

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