Abstract: AIM: To compare the effect of three different inner diameter silver clips on 2K1C hypertensive rats.METHODS: A 2K1C hypertensive rats were established by narrow the right renal artery with different inner diameter of U-shaped silver clips. Animals were divided into 4 groups: the sham-operate group, 2K1C-0.2 mm group, 2K1C-0.25 mm group and 2K1C-0.3 mm group. The tail-cuff apparatus was employed to measure the rat tail arterial blood pressure during the 0-8 weeks. At the end of 8^th wk, the hemodynamics index recorded. The cardiac and kidney hypertrophy index were expressed as heart weight/body weight (HW/BW), left kidney weight/body weight (LKW/BW) and right kidney weight/body weight (RKW/BW). The aorta remodeling was investigated by HE stain. The content of hydroxyproline in myocardial tissue was measured by colorimetric determination. Radioimmunoassay method was used to detected the AngII content in serum.RESULTS: The 2K1C-0.25 group and 2K1C-0.3 group showed the higher model successful rate, less pyknotic kidneys, significant impairment of cardiac function, markedly increasing of HW/BW, vascular remodeling index, the content of hydroxyproline and the AngII content compared with sham-operate group. However, the 2K1C-0.2 group performed not well in model successful rate, pyknotic kidneys and the AngII content in serum.CONCLUSION: The 2K1C renovascular hypertension model that narrow the renal artery with 0.25 or 0.3 mm inner diameter of U-shaped silver clips proves to be the optimal one.

Key words: Hypertension, Renin-angiotensin system, Inner diameter of silver clip, 2K1C renovascular hypertension model