Effect of Ketotifen on pancreatic function of streptozotocin-induced type 2 diabetic rats

Abstract

Abstract: AIM: To investigate the effect and mechanism of Ketotifen on pancreatic function of streptozotocin-induced type 2 diabetic rats.METHODS: Male SD rats were fed with high-carbon hydrate-fat diet 6 weeks and ip given STZ to induce a type 2 diabetes model. Rats of Ketotifen group were fed with Ketotifen 0.09 mg·kg^-1·d^-1, and killed in batches in different time (0, 4^th, 8^th week) to test the fasting blood glucose (FBG), fasting plasma insulin (FINS), the insulin sensitivity index (ISI) and homeostasis model assessment insulin resistance index (HOMA-IR) were calculated, the levels of Interleukin-6 (IL-6), tumor nectosis factor-alpha (TNF-α) were tested, and the pancreatic tissue were excised and observed by light microscope (LM) and transmission electron microscope, islet cell mitochondria were separated to test the activity of cytochrome C oxidase (CCO) and succinate dehydrogenase (SDH).RESULTS: Ketotifen suppressed serum glucose in diabetic rats (P<0.05), increased ISI and reduced HOMA-IR (P<0.01). Ketotifen reduced serum IL-6,TNF-α, increased the activity of CCO and SDH. Furthermore, Ketotifen can improve the morphological structure of islet cell.CONCLUSION: Ketotifen can improve the pancreatic function of streptozotocin-induced type 2 diabetic rats possibly by inhibition of activation of NF-κB to anti-inflammatory and improve the energy metabolism of βcell mitochondria.

Key words: Ketotifen, Diabetes mellitus, Pancreatic function, Blood glucose

CLC Number:

R285.5
965.2

CHEN Zi-miao, CHEN Xiao-jun, WANG Mei-rong, PAN Liang-liang, HU Wan-li, NI Lian-song. Effect of Ketotifen on pancreatic function of streptozotocin-induced type 2 diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(6): 627-632.

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