Differences in anxiety-related behavior induced by mCPP and responses to diazepam in two strains of mice

Abstract

Abstract: AIM: To compare the differences in anxiety-related behavior induced by chlorophenylpiperazinehydrochloride (mCPP) and response to diazepam in BALB/c mice and ICR mice.METHODS: Each were divided into four groups: saline group, mCPP group, diazepam group and (diazepam+mCPP) group. Diazepam was administrated intraperitoneally and mCPP was administrated subcutaeously 30 min before behavioral test. Open-field test, light-dark transition test and elevated plus maze test were used.RESULTS: In open-field test, the central distance ratio and the central time ratio were increased in the (diazepam+mCPP) group of BALB/c mice. But there were no effects on ICR mice.Only was there significant difference on total distance between two mice strains.In light-dark transition test, latency into dark box and number of transitions were increased, and time ratio in dark box was decreased in (diazepam+mCPP) group of two strains mice. The response to diazepam was were more sensitive in BALB/c mice than ICR mice. Number of open-arm entries, time and exploring time ratio in open-arm in the elevated plus maze all increased in (diazepam+mCPP) group of two strains mice. There were differences of time and exploring time in open-arm between them.CONCLUSION: The response to diazepam is more sensitive in anxiety mice than normal mice.BALB/c mice have low level of anxiety, high baseline exploration and risk assessment of a new environment, which is a suitable mice strain to develop anxiolytic drugs.

Key words: Anxiety, Strain difference, Diazepam, Mice, Chlorophenylpiperazinehydrochloride (mCPP)

CLC Number:

R965.2

YAN Li-ping, ZHANG Li-quan, YI Hui-min, ZHOU Xue-jun, WANG Jue, ZHANG Qi, YE Yi-lu. Differences in anxiety-related behavior induced by mCPP and responses to diazepam in two strains of mice[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(11): 1238-1243.

References

[1] Miller SM, Piasecki CC,Lonstein JS. Use of the light-dark box to compare the anxiety-related behavior of virgin and postpartum female rats[J]. Pharmacol Biochem Behav, 2011, 100(1): 130-137.
[2] Chatterjee M, Verma R, Lakshmi V, et al. Anxiolytic effects of plumeria rubra var. acutifolia (Poiret) L. flower extracts in the elevated Plus-maze model of anxiety in mice[J]. Asian J Psychiatr, 2013, 6(2): 113-118.
[3] Enkel T, Thomas M.Bartsch D. Differential effects of subchronic phencyclidine on anxiety in the light-enhanced startle-, light/dark exPloration- and open field tests[J]. Behav Brain Res, 2013, 243(5):61-65.
[4] Hajikhani R, Ahmadi A, Naderi N, et al. Effect of phencyclidine derivatives on anxiety-like behavior using an elevated-plus maze test in mice[J]. Adv Clin Exp Med, 2013, 21(3): 307-312.
[5] van Gaalen MM, Steckler T. Behavioural analysis of four mouse strains in an anxiety test battery[J]. Behav Brain Res, 2000, 115(1): 95-106.
[6] Roman E, Meyerson BJ, Hyytia P, et al. The multivariate concentric square field test reveals different behavioural profiles in male AA and ANA rats with regard to risk taking and environmental reactivity[J]. Behav Brain Res, 2007, 183(2): 195-205.
[7] Bagdy G, Graf M, Anheuer ZE, et al. Anxiety-like effects induced by acute fluoxetine, sertraline or mCPP treatment are reversed by pretreatment with the 5-HT2C receptor antagonist SB-242084 but not the 5-HT1A receptor antagonist WAY-100635[J]. Int J Neuropsychopharmacol, 2001, 4(4): 399-408.
[8] Mamiya T, Asanuma T, Kawai Y, et al. Effects of soybean food pellets on mCPP-induced anxiety model of mice[J]. Biol Pharm Bull, 2006, 29(7): 1498-1500.
[9] Peng WH, Hsieh MT, Lee YS, et al. Anxiolytic effect of seed of Ziziphus jujuba in mouse models of anxiety[J]. J Ethnopharmacol, 2000, 72(3): 435-441.
[10] Van Veen JF, Van der Wee NJ, Fiselier J, et al. Behavioural effects of rapid intravenous administration of meta-chlorophenylpiperazine (mCPP) in patients with generalized social anxiety disorder, panic disorder and healthy controls[J]. Eur Neuropsychopharmacol, 2007, 17(10): 637-642.
[11] Yokoyama F, Yamauchi M, Oyama M, et al. Anxiolytic-like profiles of histamine H3 receptor agonists in animal models of anxiety: a comparative study with antidepressants and benzodiazepine anxiolytic[J]. Psychopharmacology (Berl), 2009, 205(2): 177-187.
[12] Chapouthier G, Bondoux D, Martin B, et al. Genetic difference in sensitivity to beta-carboline: evidence for the involvement of brain benzodiazepine receptors[J]. Brain Res, 1991, 553(2): 342-346.
[13] Lepicard EM, Joubert C, Hagneau I, et al. Differences in anxiety-related behavior and response to diazepam in BALB/cByJ and C57BL/6J strains of mice[J]. Pharmacol Biochem Behav, 2000, 67(4): 739-748.
[14] Cao BJ. Rodgers RJ. Comparative effects of novel 5-HT1A receptor ligands, LY293284, LY315712 and LY297996, on plus-maze anxiety in mice[J]. Psychopharmacology (Berl), 1998, 139(3): 185-194.
[15] 童九翠,谢海棠,杨斌,等. 豆腐果苷对慢性应激小鼠行为学的影响[J]. 中国临床药理学与治疗学,2010,15(6):641-645.
[16] Zhang Q, Yu YP, Ye YL, et al. Spatiotemporal properties of locomotor activity after administration of central nervous stimulants and sedatives in mice[J]. Pharmacol Biochem Behav, 2011, 97(3): 577-585.
[17] Robertson HA. Benzodiazepine receptors in “emotional” and “non-emotional” mice; comparison of four strains[J]. Eur J Pharmacol, 1979, 56(1/2): 163-166.
[18] An XL, Zou JX, Wu RY, et al. Strain and sex differences in anxiety-like and social behaviors in C57BL/6J and BALB/cJ mice[J]. Exp Anim, 2011, 60(2): 111-123.
[19] Costall B, Jones BJ, Kelly ME, et al. Exploration of mice in a black and white test box: validation as a model of anxiety[J]. Pharmacol Biochem Behav, 1989, 32(3): 777-785.
[20] Baptista D, Nunes-de-Souza RL, Canto-de-Souza A. Activation of 5-HT(2C) receptors in the dorsal periaqueductal gray increases antinociception in mice exposed to the elevated plus-maze[J]. Behav Brain Res, 2012, 235(1): 42-47.