Interference of shRNA on survivin attenuate cellular DNA damage repair induced by ultraviolet radiation

Abstract

Abstract: AIM: To detect the DNA damage repair effect and its mechanism mediated by survivin during UV-induced apoptosis in tumor cells.METHODS: This study constructed and packaged lentiviral vector for survivin shRNA. Transfection efficiency and titer were measured by the dilution method. Real-time fluorescence quantitative method was applied to identify interference effects. Host-cells reactivating assay (HCR) was adopted to evaluate impact of survivin on cellular DNA damage repair capacity.RESULTS: Interference of survivin significantly reduced cellular DNA damage repair on lung adenocarcinoma H1299.CONCLUSION: Survivin plays an important role in DNA damage repair. Our research provides evidence for cancer therapy targeting survivin.

Key words: survivin, UV, tumor cell, DNA damage repair

CLC Number:

R965.2

SHEN Jie, CHU Ji-ru, JIA Yuan-wei, LIU Xiao-ping. Interference of shRNA on survivin attenuate cellular DNA damage repair induced by ultraviolet radiation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(5): 497-502.

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