Abstract: AIM: To investigate the effects of luoxianzi extractive on the adherence rate of monocyte and vascular endothelial cell. METHODS: The luoxianzi extractive which molecular weight was less than 30 000 was prepared; the influence of luoxianzi extract on vascular endothelial cells EA.hy926's vitality was investigated; the THP-1 and EA.hy926 adhesion model induced by tumor necrosis factor (TNF-α) was built,using rose bengal staining, investigated the different concentrations of luoxianzi extractive on the adherence effect of THP-1 and EA.hy926; the influence of luoxianzai extract on EA.hy926 secreted MCP-1 and VCAM-1 which induced by TNF-α was investigated by ELISA.RESULTS:Luoxianzi extract (concentration≤4 × 10² μg/mL) had no significant inhibition or proliferation effect in EA.hy926(P>0.05); luoxianzi extract (concentration≥6.25 μg/mL) could concentration-dependent decrease the adhesion between two cells; all concentrations of luoxianzi extract could significantly reduce the MCP-1 expression induced by TNF-α (P<0.01), luoxianzi extract (concentration ≥ 50 μg/mL), significantly reduced the VCAM-1 expression induced by TNF-α. CONCLUSION: Luoxianzi extract can reduce TNF-α-induced endothelial cells to secrete MCP-1 and VCAM-1, blocking monocyte adherence to vascular endothelial cells, and thus play the role of anti-atherosclerosis.

Key words: luoxianzi, atherosclerosis, adhesiveness, vascular endothelial cell