Effect of human umbilical vein endothelial cells apoptosis induced by ethanol and the expression of ICAM-1

Abstract

Abstract: AIM: To study the apoptosis induced by ethanol on human umbilical vein endothelial cells(HUVEC)and the expression of ICAM-1 in vitro.METHODS: HUVEC were treated with different concentrations of ethanol (50,100 and 200 mmol/L) for 24 hours, the activity of cells was determined by methyl thiazolyl tetrazolium (MTT) assay; the rate of apoptosis was detected by flow cytometry (FCM) assay; and the expression of ICAM-1 by enzyme linked immunosorbent assay.RESULTS: Ethanol can inhibit the cells activity, promote the apoptosis, increase the expression of ICAM-1 of HUVEC in dose-dependent manner.CONCLUSION: Ethanol can damage endothelial cells and its possible mechanism may be linked to promote the apoptosis and the expression of ICAM-1.

Key words: ethanol, HUVEC, ICAM-1, apoptosis

CLC Number:

R965.2

HE Sheng-hu, ZHOU Sheng-hua, ZHANG Jing, WANG Xue-fei, WANG Da-xin. Effect of human umbilical vein endothelial cells apoptosis induced by ethanol and the expression of ICAM-1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 266-268.

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